Journal article
Increase in lens capsule stiffness caused by vital dyes.
- Haritoglou C From the Department of Ophthalmology (Haritoglou, Schumann, Wolf, Kernt) and the Department of Applied Physics and Center for NanoScience (Mauell, Benoit), Ludwig-Maximilians-University, Munich, Germany; the Department of Ophthalmology (Henrich), University Hospital Basel, Basel, Switzerland. Electronic address: christos.haritoglou@med.uni-muenchen.de.
- Mauell S
- Schumann RG
- Henrich PB
- Wolf A
- Kernt M
- Benoit M
- 2013-09-10
Published in:
- Journal of cataract and refractive surgery. - 2013
Cataract Extraction
Coloring Agents
Elasticity
Humans
Indocyanine Green
Lens Capsule, Crystalline
Light
Microscopy, Atomic Force
Rosaniline Dyes
Staining and Labeling
Trypan Blue
English
PURPOSE
To assess potential changes in lens capsule mechanical properties after staining with brilliant blue, indocyanine green (ICG), and trypan blue.
SETTING
Department of Ophthalmology and Applied Physics and Center for NanoScience, Ludwig-Maximilians-University, Munich, Germany.
DESIGN
Experimental study.
METHODS
Fifteen unstained lens capsules were dissected into 7 wedge-shaped parts. Three fragments were stained with brilliant blue 0.025%, ICG 0.05%, and trypan blue 0.06%, respectively, for 1 minute. Another 3 specimens were additionally illuminated using a standard light source. The seventh part served as an untreated control. All specimens were analyzed using atomic force microscopy (AFM) in contact mode with a scan rate of 0.6 Hz. Two scan regions of 10 μm × 10 μm were chosen, and stiffness was determined using AFM in a force spectroscopy mode. The force curves were performed with a data rate of 5000 Hz.
RESULTS
Staining of the samples resulted in an increase in tissue stiffness (brilliant blue: P<.001; ICG: P<.01; trypan blue: P<.05). Additional illumination after staining further increased tissue stiffness, but not significantly. Mean increase in the relative elasticity values were 1.61 ± 0.15 (SD) for brilliant blue, 2.04 ± 0.21 for brilliant blue with illumination, 1.63 ± 0.22 for ICG, 2.01 ± 0.22 for ICG with illumination, 1.23 ± 0.11 for trypan blue, and 1.39 ± 0.11 for trypan blue with illumination. In relation to unstained tissue, the relative elasticity of the stained tissue increased 1.2-fold after illumination.
CONCLUSION
Staining significantly increased the mechanical properties of the human lens capsule.
FINANCIAL DISCLOSURE
No author has a financial or proprietary interest in any material or method mentioned.
To assess potential changes in lens capsule mechanical properties after staining with brilliant blue, indocyanine green (ICG), and trypan blue.
SETTING
Department of Ophthalmology and Applied Physics and Center for NanoScience, Ludwig-Maximilians-University, Munich, Germany.
DESIGN
Experimental study.
METHODS
Fifteen unstained lens capsules were dissected into 7 wedge-shaped parts. Three fragments were stained with brilliant blue 0.025%, ICG 0.05%, and trypan blue 0.06%, respectively, for 1 minute. Another 3 specimens were additionally illuminated using a standard light source. The seventh part served as an untreated control. All specimens were analyzed using atomic force microscopy (AFM) in contact mode with a scan rate of 0.6 Hz. Two scan regions of 10 μm × 10 μm were chosen, and stiffness was determined using AFM in a force spectroscopy mode. The force curves were performed with a data rate of 5000 Hz.
RESULTS
Staining of the samples resulted in an increase in tissue stiffness (brilliant blue: P<.001; ICG: P<.01; trypan blue: P<.05). Additional illumination after staining further increased tissue stiffness, but not significantly. Mean increase in the relative elasticity values were 1.61 ± 0.15 (SD) for brilliant blue, 2.04 ± 0.21 for brilliant blue with illumination, 1.63 ± 0.22 for ICG, 2.01 ± 0.22 for ICG with illumination, 1.23 ± 0.11 for trypan blue, and 1.39 ± 0.11 for trypan blue with illumination. In relation to unstained tissue, the relative elasticity of the stained tissue increased 1.2-fold after illumination.
CONCLUSION
Staining significantly increased the mechanical properties of the human lens capsule.
FINANCIAL DISCLOSURE
No author has a financial or proprietary interest in any material or method mentioned.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1016/j.jcrs.2013.02.057
- PMID 24011931
- Persistent URL
- https://sonar.ch/global/documents/232136
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