Visualizing the spatiotemporal dynamics of DNA damage in budding yeast.

Horigome C; Dion V; Seeber A; Gehlen LR; Gasser SM

doi:10.1007/978-1-4939-2522-3_6

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Journal article

Visualizing the spatiotemporal dynamics of DNA damage in budding yeast.

Horigome C Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, 4058, Basel, Switzerland.
Dion V
Seeber A
Gehlen LR
Gasser SM

2015-03-26

Published in:

Methods in molecular biology (Clifton, N.J.). - 2015

English Fluorescence microscopy has enabled the analysis of both the spatial distribution of DNA damage and its dynamics during the DNA damage response (DDR). Three microscopic techniques can be used to study the spatiotemporal dynamics of DNA damage. In the first part we describe how we determine the position of DNA double-strand breaks (DSBs) relative to the nuclear envelope. The second part describes how to quantify the co-localization of DNA DSBs with nuclear pore clusters, or other nuclear subcompartments. The final protocols describe methods for the quantification of locus mobility over time.

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