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A 3D printed microfluidic device for production of functionalized hydrogel microcapsules for culture and differentiation of human Neuronal Stem Cells (hNSC).

  • Alessandri K University of Geneva, Department of Biochemistry, quai Ernest Ansermet 30, CH-1211 Geneva 4, Switzerland. aurelien.roux@unige.ch and Institut Curie et Centre National de la Recherche Scientifique, Unité Mixte de Recherche 168, F-75248 Paris, France and Université Pierre et Marie Curie, F-75005 Paris, France.
  • Feyeux M Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, CH-1211 Geneva 4, Switzerland.
  • Gurchenkov B Institut Curie et Centre National de la Recherche Scientifique, Unité Mixte de Recherche 168, F-75248 Paris, France and Université Pierre et Marie Curie, F-75005 Paris, France and ICI, IGBMC, CNRS, UMR7104, F-67404 Illkirch-Graffenstaden, France and INSERM, U964, Université de Strasbourg, F-67400 Illkirch-Graffenstaden, France and Institut Curie et Centre National de la Recherche Scientifique, Unité Mixte de Recherche 144, F-75248 Paris, France.
  • Delgado C Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, CH-1211 Geneva 4, Switzerland.
  • Trushko A University of Geneva, Department of Biochemistry, quai Ernest Ansermet 30, CH-1211 Geneva 4, Switzerland. aurelien.roux@unige.ch.
  • Krause KH Department of Pathology and Immunology, Faculty of Medicine, University of Geneva, CH-1211 Geneva 4, Switzerland.
  • Vignjević D Institut Curie et Centre National de la Recherche Scientifique, Unité Mixte de Recherche 144, F-75248 Paris, France.
  • Nassoy P University of Geneva, Department of Biochemistry, quai Ernest Ansermet 30, CH-1211 Geneva 4, Switzerland. aurelien.roux@unige.ch and Institut Curie et Centre National de la Recherche Scientifique, Unité Mixte de Recherche 168, F-75248 Paris, France and Université de Bordeaux, LP2N, UMR 5298, F-33400 Talence, France and Institut d'Optique & CNRS, LP2N, UMR 5298, F-33400 Talence, France.
  • Roux A University of Geneva, Department of Biochemistry, quai Ernest Ansermet 30, CH-1211 Geneva 4, Switzerland. aurelien.roux@unige.ch and Swiss National Centre for Competence in Research Programme Chemical Biology, 1211 Geneva, Switzerland.
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  • 2016-03-31
Published in:
  • Lab on a chip. - 2016
Alginates
Automation, Laboratory
Cell Culture Techniques
Cell Line
Cell Survival
Cells, Immobilized
Cellular Microenvironment
Collagen
Drug Combinations
Extracellular Matrix
Glucuronic Acid
Hexuronic Acids
High-Throughput Screening Assays
Humans
Hydrogels
Induced Pluripotent Stem Cells
Lab-On-A-Chip Devices
Laminin
Nerve Tissue Proteins
Neural Stem Cells
Neurogenesis
Neurons
Printing, Three-Dimensional
Proof of Concept Study
Proteoglycans
Stem Cell Niche
Surface Properties
English We present here a microfluidic device that generates sub-millimetric hollow hydrogel spheres, encapsulating cells and coated internally with a layer of reconstituted extracellular matrix (ECM) of a few microns thick. The spherical capsules, composed of alginate hydrogel, originate from the spontaneous instability of a multi-layered jet formed by co-extrusion using a coaxial flow device. We provide a simple design to manufacture this device using a DLP (digital light processing) 3D printer. Then, we demonstrate how the inner wall of the capsules can be decorated with a continuous ECM layer that is anchored to the alginate gel and mimics the basal membrane of a cellular niche. Finally, we used this approach to encapsulate human Neural Stem Cells (hNSC) derived from human Induced Pluripotent Stem Cells (hIPSC), which were further differentiated into neurons within the capsules with negligible loss of viability. Altogether, we show that these capsules may serve as cell micro-containers compatible with complex cell culture conditions and applications. These developments widen the field of research and biomedical applications of the cell encapsulation technology.
Language
  • English
Open access status
green
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https://sonar.ch/global/documents/232427
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