Cryo-EM analysis of homodimeric full-length LRRK2 and LRRK1 protein complexes.
- Sejwal K Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Basel, 4056, Switzerland.
- Chami M Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Basel, 4056, Switzerland.
- Rémigy H FEI Company, Eindhoven, The Netherlands.
- Vancraenenbroeck R KU Leuven, Laboratory for Neurobiology and Gene Therapy, Department of Neurosciences, 3000, Leuven, Belgium.
- Sibran W Université de Lille, Inserm, CHU Lille, UMR-S1172 - JPArc - Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer, F-59000, Lille, France.
- Sütterlin R Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Basel, 4056, Switzerland.
- Baumgartner P Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Basel, 4056, Switzerland.
- McLeod R Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Basel, 4056, Switzerland.
- Chartier-Harlin MC Université de Lille, Inserm, CHU Lille, UMR-S1172 - JPArc - Centre de Recherche Jean-Pierre AUBERT Neurosciences et Cancer, F-59000, Lille, France.
- Baekelandt V KU Leuven, Laboratory for Neurobiology and Gene Therapy, Department of Neurosciences, 3000, Leuven, Belgium.
- Stahlberg H Center for Cellular Imaging and NanoAnalytics (C-CINA), Biozentrum, University of Basel, Basel, 4056, Switzerland. henning.stahlberg@unibas.ch.
- Taymans JM KU Leuven, Laboratory for Neurobiology and Gene Therapy, Department of Neurosciences, 3000, Leuven, Belgium. jean-marc.taymans@inserm.fr.
- 2017-08-19
Published in:
- Scientific reports. - 2017
Buffers
Cryoelectron Microscopy
Detergents
Humans
Imaging, Three-Dimensional
Leucine-Rich Repeat Serine-Threonine Protein Kinase-2
Protein Folding
Protein Multimerization
Protein-Serine-Threonine Kinases
Solutions
English
Leucine-rich repeat kinase 2 (LRRK2) is a large multidomain protein implicated in the pathogenesis of both familial and sporadic Parkinson's disease (PD), and currently one of the most promising therapeutic targets for drug design in Parkinson's disease. In contrast, LRRK1, the closest homologue to LRRK2, does not play any role in PD. Here, we use cryo-electron microscopy (cryo-EM) and single particle analysis to gain structural insight into the full-length dimeric structures of LRRK2 and LRRK1. Differential scanning fluorimetry-based screening of purification buffers showed that elution of the purified LRRK2 protein in a high pH buffer is beneficial in obtaining high quality cryo-EM images. Next, analysis of the 3D maps generated from the cryo-EM data show 16 and 25 Å resolution structures of full length LRRK2 and LRRK1, respectively, revealing the overall shape of the dimers with two-fold symmetric orientations of the protomers that is closely similar between the two proteins. These results suggest that dimerization mechanisms of both LRRKs are closely related and hence that specificities in functions of each LRRK are likely derived from LRRK2 and LRRK1's other biochemical functions. To our knowledge, this study is the first to provide 3D structural insights in LRRK2 and LRRK1 dimers in parallel.
- Language
-
- English
- Open access status
- gold
- Identifiers
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- DOI 10.1038/s41598-017-09126-z
- PMID 28819229
- Persistent URL
- https://sonar.ch/global/documents/232472
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