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In-capillary immuno-preconcentration with circulating bio-functionalized magnetic beads for capillary electrophoresis.

  • Mai TD Institut Galien Paris Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Univ. Paris-Saclay, 5 rue Jean Baptiste Clément, 92290, Châtenay-Malabry, France. Electronic address: thanh-duc.mai@u-psud.fr.
  • Hauser PC University of Basel, Department of Chemistry, Spitalstrasse 51, 4056, Basel, Switzerland.
  • Descroix S Laboratoire Physico Chimie Curie, Institut Curie, PSL Research University, CNRS UMR168, 75005, Paris, France.
  • Crosnier de Lassichère C Institut Galien Paris Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Univ. Paris-Saclay, 5 rue Jean Baptiste Clément, 92290, Châtenay-Malabry, France.
  • Taverna M Institut Galien Paris Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Univ. Paris-Saclay, 5 rue Jean Baptiste Clément, 92290, Châtenay-Malabry, France.
  • Smadja C Institut Galien Paris Sud, UMR 8612, Protein and Nanotechnology in Analytical Science (PNAS), CNRS, Univ. Paris-Sud, Univ. Paris-Saclay, 5 rue Jean Baptiste Clément, 92290, Châtenay-Malabry, France.
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  • 2019-04-06
Published in:
  • Analytica chimica acta. - 2019
Alzheimer's disease
Amyloid peptide beta
Capillary electrophoresis
Circulating magnetic beads
Magneto-immunocapture
Alzheimer Disease
Amyloid beta-Peptides
Electrophoresis, Capillary
Humans
Immunomagnetic Separation
Magnetic Fields
Peptide Fragments
English This study reports on the conception of magneto-Capillary Electrophoresis (magneto-CE), an approach integrating immuno-capture on circulating bio-functionalized magnetic beads into a unique capillary for preconcentration and electrokinetic separation. This hybrid mode is an evolution of in-capillary magnetic bead-based operation from static cluster format to dynamic configuration where beads are allowed to controllably circulate inside a CE capillary for interaction improvement. To implement the magneto-CE operation, a purpose-made instrument was constructed, allowing visual observation of the movement of the magnetic beads. We applied a new methodological strategy for determination of the amyloid β peptide (Aβ 1-42), which is as an established biomarker for molecular diagnosis of Alzheimer's disease (AD). The methodology is based on magneto-immuno-capture of fluorescently labeled Aβ 1-42 followed by a chemical elution with a basic solution prior to CE separation with laser induced fluorescent (LIF) detection. The superiority of this dynamic configuration of magneto-CE was demonstrated for this target analyte, with sample pretreatment and separation being performed in-capillary without any delay in between and without any waste of pretreated sample, which otherwise would not be the case with offline/batch-wise operation.
Language
  • English
Open access status
green
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https://sonar.ch/global/documents/232496
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