Journal article
Hoechst 33258 as a vital stain for plant cell protoplasts.
- Meadows MG Friedrich Miescher-Institut, Postfach 273, CH-4002, Basel, Switzerland.
- Potrykus I
- 2013-11-22
Published in:
- Plant cell reports. - 1981
English
A method for staining nuclei in unfixed protoplasts using Hoechst 33258 is described. It was found that the dye was not taken up by all protoplasts except in the presence of a detergent and that the staining of the nuclei was pH- and concentration-dependent. The use of vital fluorescent probes with plant protoplasts may be helpful for the identification, selection and subsequent culture of hybrid fusion products in automatic cell sorting systems.
- Language
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- English
- Open access status
- closed
- Identifiers
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- DOI 10.1007/BF00269279
- PMID 24258866
- Persistent URL
- https://sonar.ch/global/documents/232756
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