Biotransformation of substituted benzoates to the corresponding cis-diols by an engineered strain of Pseudomonas oleovorans producing the TOL plasmid-specified enzyme toluate-1,2-dioxygenase.
- Wubbolts MG Department of Medical Biochemistry, Faculty of Medicine, University of Geneva, Switzerland.
- Timmis KN
- 1990-02-01
Published in:
- Applied and environmental microbiology. - 1990
Benzoates
Biotransformation
Gene Expression Regulation, Bacterial
Genetic Engineering
Oxygenases
Plasmids
Pseudomonas
English
The conversion of substituted benzoates into 1,2-cis-dihydroxycyclohexa-3,5-diene carboxylic acids (cis-diols) was effected by using Escherichia coli and Pseudomonas recombinants carrying the xylXYZ genes originating from the Pseudomonas putida mt-2 TOL plasmid, thus producing toluate-1,2-dioxygenase. Pseudomonas oleovorans GPo12 recombinants readily produced meta- and para-substituted cis-diols, but were limited in their oxidation of ortho-substituted substrates.
- Language
-
- English
- Open access status
- bronze
- Identifiers
-
- DOI 10.1128/AEM.56.2.569-571.1990
- PMID 2306096
- Persistent URL
- https://sonar.ch/global/documents/232888
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