Back
Undressing of Waddlia chondrophila to enrich its outer membrane proteins to develop a new species-specific ELISA.
Journal article

Undressing of Waddlia chondrophila to enrich its outer membrane proteins to develop a new species-specific ELISA.

  • Lienard J Center for Research on Intracellular Bacteria, Lausanne University Hospital and University of Lausanne Lausanne.
  • Croxatto A Center for Research on Intracellular Bacteria, Lausanne University Hospital and University of Lausanne Lausanne.
  • Gervaix A Children's Hospital of Geneva, University Hospitals of Geneva and Medical School of the University of Geneva Switzerland.
  • Posfay-Barbe K Children's Hospital of Geneva, University Hospitals of Geneva and Medical School of the University of Geneva Switzerland.
  • Baud D Center for Research on Intracellular Bacteria, Lausanne University Hospital and University of Lausanne Lausanne ; St Mary's Hospital London, United Kingdom.
  • Kebbi-Beghdadi C Center for Research on Intracellular Bacteria, Lausanne University Hospital and University of Lausanne Lausanne.
  • Greub G Center for Research on Intracellular Bacteria, Lausanne University Hospital and University of Lausanne Lausanne.
Show more…
  • 2014-10-31
Published in:
  • New microbes and new infections. - 2014
Chlamydia-like organisms
Waddlia chondrophila
intracellular bacteria
mass spectrometry
miscarriage
serology
English Waddlia chondrophila, an obligate intracellular bacterium of the Chlamydiales order, is considered as an agent of bovine abortion and a likely cause of miscarriage in humans. Its role in respiratory diseases was questioned after the detection of its DNA in clinical samples taken from patients suffering from pneumonia or bronchiolitis. To better define the role of Waddlia in both miscarriage and pneumonia, a tool allowing large-scale serological investigations of Waddlia seropositivity is needed. Therefore, enriched outer membrane proteins of W. chondrophila were used as antigens to develop a specific ELISA. After thorough analytical optimization, the ELISA was validated by comparison with micro-immunofluorescence and it showed a sensitivity above 85% with 100% specificity. The ELISA was subsequently applied to human sera to specify the role of W. chondrophila in pneumonia. Overall, 3.6% of children showed antibody reactivity against W. chondrophila but no significant difference was observed between children with and without pneumonia. Proteomic analyses were then performed using mass spectrometry, highlighting members of the outer membrane protein family as the dominant proteins. The major Waddlia putative immunogenic proteins were identified by immunoblot using positive and negative human sera. The new ELISA represents an efficient tool with high throughput applications. Although no association with pneumonia and Waddlia seropositivity was observed, this ELISA could be used to specify the role of W. chondrophila in miscarriage and in other diseases.
Language
  • English
Open access status
gold
Identifiers
Persistent URL
https://sonar.ch/global/documents/232891
Statistics
Document views: 40 File downloads: