Journal article
Cell-free nucleic acids are present in blood products and regulate genes of innate immune response.
- Waldvogel Abramowski S Academic Department of Medical Specialties, Geneva University Hospitals, Geneva, Switzerland.
- Tirefort D Academic Department of Medical Specialties, Geneva University Hospitals, Geneva, Switzerland.
- Lau P Academic Department of Medical Specialties, Geneva University Hospitals, Geneva, Switzerland.
- Guichebaron A Academic Department of Medical Specialties, Geneva University Hospitals, Geneva, Switzerland.
- Taleb S Academic Department of Medical Specialties, Geneva University Hospitals, Geneva, Switzerland.
- Modoux C Laboratory of Clinical Immunology and Allergy, Geneva University Hospitals, Geneva, Switzerland.
- Lemoine Chaduc C Academic Department of Medical Specialties, Geneva University Hospitals, Geneva, Switzerland.
- Bruyere Cerdan P Laboratory of Clinical Immunology and Allergy, Geneva University Hospitals, Geneva, Switzerland.
- Roux Lombard P Laboratory of Clinical Immunology and Allergy, Geneva University Hospitals, Geneva, Switzerland.
- Lecompte T Academic Department of Medical Specialties, Geneva University Hospitals, Geneva, Switzerland.
- Preynat-Seauve O Academic Department of Medical Specialties, Geneva University Hospitals, Geneva, Switzerland.
- 2018-04-18
Published in:
- Transfusion. - 2018
English
BACKGROUND
Extracellular nucleic acids circulate in plasma. They are expected to be present in manufactured blood products eligible for transfusion, but little is known about their biological activity on human cells. The aim of this study is to investigate whether cell-free nucleic acids (CFNAs) are present and biologically active in red blood cell units (RBCUs), fresh frozen plasmas, and platelet concentrates.
STUDY DESIGN AND METHODS
CFNAs were extracted from RBCUs, fresh frozen plasma, and platelet concentrates. Their nature and structure were analyzed by regular methods of nucleic acid detection/quantification. A normalized polymerase chain reaction combining amplification of a CFNA marker (Alu 115) and amplification of an internal nonhuman DNA control spiked in all samples (phiX 174) was developed to study CFNA release after RBCU storage. The impact of CFNAs on gene regulation was tested by microarray after coculture with peripheral blood mononuclear cells and macrophages.
RESULTS
Extracellular double-stranded DNA was present in all blood products, with higher amounts found in cellular suspensions (RBCUs and platelet concentrates). Storage up to 40 days did not influence release from RBCUs, and CFNA amount varied considerably from one unit to another. Microarray experiments showed that exposition of macrophages to CFNA increased the expression of genes involved in the innate immune response including chemokines, chemokine receptors, and receptors of the innate response.
CONCLUSION
CFNAs are present in blood products. Immunoregulatory properties of CFNA are shown in vitro, providing new insights on biologically active components of blood products besides those for intended therapeutic use.
Extracellular nucleic acids circulate in plasma. They are expected to be present in manufactured blood products eligible for transfusion, but little is known about their biological activity on human cells. The aim of this study is to investigate whether cell-free nucleic acids (CFNAs) are present and biologically active in red blood cell units (RBCUs), fresh frozen plasmas, and platelet concentrates.
STUDY DESIGN AND METHODS
CFNAs were extracted from RBCUs, fresh frozen plasma, and platelet concentrates. Their nature and structure were analyzed by regular methods of nucleic acid detection/quantification. A normalized polymerase chain reaction combining amplification of a CFNA marker (Alu 115) and amplification of an internal nonhuman DNA control spiked in all samples (phiX 174) was developed to study CFNA release after RBCU storage. The impact of CFNAs on gene regulation was tested by microarray after coculture with peripheral blood mononuclear cells and macrophages.
RESULTS
Extracellular double-stranded DNA was present in all blood products, with higher amounts found in cellular suspensions (RBCUs and platelet concentrates). Storage up to 40 days did not influence release from RBCUs, and CFNA amount varied considerably from one unit to another. Microarray experiments showed that exposition of macrophages to CFNA increased the expression of genes involved in the innate immune response including chemokines, chemokine receptors, and receptors of the innate response.
CONCLUSION
CFNAs are present in blood products. Immunoregulatory properties of CFNA are shown in vitro, providing new insights on biologically active components of blood products besides those for intended therapeutic use.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1111/trf.14613
- PMID 29664127
- Persistent URL
- https://sonar.ch/global/documents/232930
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