Quantitation of alpha-factor internalization and response during the Saccharomyces cerevisiae cell cycle.
- Zanolari B Biocenter, University of Basel, Switzerland.
- Riezman H
- 1991-10-01
Published in:
- Molecular and cellular biology. - 1991
Cell Cycle
Endocytosis
Gene Expression Regulation, Fungal
Genes, Fungal
Kinetics
Mating Factor
Microscopy, Fluorescence
Peptides
Receptors, Cell Surface
Receptors, Mating Factor
Receptors, Peptide
Saccharomyces cerevisiae
Signal Transduction
Transcription Factors
English
The alpha-factor pheromone binds to specific cell surface receptors on Saccharomyces cerevisiae a cells. The pheromone is then internalized, and cell surface receptors are down-regulated. At the same time, a signal is transmitted that causes changes in gene expression and cell cycle arrest. We show that the ability of cells to internalize alpha-factor is constant throughout the cell cycle, a cells are also able to respond to pheromone throughout the cycle even though there is cell cycle modulation of the expression of two pheromone-inducible genes, FUS1 and STE2. Both of these genes are expressed less efficiently near or just after the START point of the cell cycle in response to alpha-factor. For STE2, the basal level of expression is modulated in the same manner.
- Language
-
- English
- Open access status
- bronze
- Identifiers
-
- DOI 10.1128/mcb.11.10.5251
- PMID 1656226
- Persistent URL
- https://sonar.ch/global/documents/233036
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