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The Ol1mpiad: concordance of behavioural faculties of stage 1 and stage 3 Drosophila larvae.

  • Almeida-Carvalho MJ Gulbenkian Institute of Science, 2780-156 Oeiras, Portugal.
  • Berh D Institute of Neurobiology and Behavioural Biology, University of Münster, 48149 Münster, Germany.
  • Braun A EMBL/CRG Systems Biology Unit, Centre for Genomic Regulation, 08003 Barcelona, Spain.
  • Chen YC Leibniz Institute for Neurobiology (Genetics), 39118 Magdeburg, Germany bertram.gerber@lin-magdeburg.de thomas.niewalda@lin-magdeburg.de yi-chun.chen@lin-magdeburg.de.
  • Eichler K Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
  • Eschbach C Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
  • Fritsch PMJ Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland.
  • Gerber B Leibniz Institute for Neurobiology (Genetics), 39118 Magdeburg, Germany bertram.gerber@lin-magdeburg.de thomas.niewalda@lin-magdeburg.de yi-chun.chen@lin-magdeburg.de.
  • Hoyer N Center for Molecular Neurobiology, University of Hamburg, 20251 Hamburg, Germany.
  • Jiang X Department of Mathematics and Computer Science, University of Münster, 48149 Münster, Germany.
  • Kleber J Leibniz Institute for Neurobiology (Genetics), 39118 Magdeburg, Germany.
  • Klämbt C Institute of Neurobiology and Behavioural Biology, University of Münster, 48149 Münster, Germany.
  • König C Leibniz Institute for Neurobiology (Molecular Systems Biology), 39118 Magdeburg, Germany.
  • Louis M EMBL/CRG Systems Biology Unit, Centre for Genomic Regulation, 08003 Barcelona, Spain.
  • Michels B Leibniz Institute for Neurobiology (Genetics), 39118 Magdeburg, Germany.
  • Miroschnikow A LIMES-Institute, University of Bonn, 53115 Bonn, Germany.
  • Mirth C Gulbenkian Institute of Science, 2780-156 Oeiras, Portugal.
  • Miura D Department of Biology, Kyushu University, 819-0395 Fukuoka, Japan.
  • Niewalda T Leibniz Institute for Neurobiology (Genetics), 39118 Magdeburg, Germany bertram.gerber@lin-magdeburg.de thomas.niewalda@lin-magdeburg.de yi-chun.chen@lin-magdeburg.de.
  • Otto N Institute of Neurobiology and Behavioural Biology, University of Münster, 48149 Münster, Germany.
  • Paisios E Leibniz Institute for Neurobiology (Genetics), 39118 Magdeburg, Germany.
  • Pankratz MJ LIMES-Institute, University of Bonn, 53115 Bonn, Germany.
  • Petersen M Center for Molecular Neurobiology, University of Hamburg, 20251 Hamburg, Germany.
  • Ramsperger N Department of Biology, University of Konstanz, 78464 Konstanz, Germany.
  • Randel N Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
  • Risse B Institute of Neurobiology and Behavioural Biology, University of Münster, 48149 Münster, Germany.
  • Saumweber T Leibniz Institute for Neurobiology (Genetics), 39118 Magdeburg, Germany.
  • Schlegel P LIMES-Institute, University of Bonn, 53115 Bonn, Germany.
  • Schleyer M Leibniz Institute for Neurobiology (Genetics), 39118 Magdeburg, Germany.
  • Soba P Center for Molecular Neurobiology, University of Hamburg, 20251 Hamburg, Germany.
  • Sprecher SG Department of Biology, University of Fribourg, 1700 Fribourg, Switzerland.
  • Tanimura T Department of Biology, Kyushu University, 819-0395 Fukuoka, Japan.
  • Thum AS Department of Biology, University of Konstanz, 78464 Konstanz, Germany.
  • Toshima N Leibniz Institute for Neurobiology (Genetics), 39118 Magdeburg, Germany.
  • Truman JW Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
  • Yarali A Center for Behavioral Brain Sciences, Otto von Guericke University Magdeburg, 39106 Magdeburg, Germany.
  • Zlatic M Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
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  • 2017-07-07
Published in:
  • The Journal of experimental biology. - 2017
Feeding
Learning and memory
Locomotion
Navigation
Sensory processing
Animals
Behavior, Animal
Brain
Drosophila melanogaster
Larva
English Mapping brain function to brain structure is a fundamental task for neuroscience. For such an endeavour, the Drosophila larva is simple enough to be tractable, yet complex enough to be interesting. It features about 10,000 neurons and is capable of various taxes, kineses and Pavlovian conditioning. All its neurons are currently being mapped into a light-microscopical atlas, and Gal4 strains are being generated to experimentally access neurons one at a time. In addition, an electron microscopic reconstruction of its nervous system seems within reach. Notably, this electron microscope-based connectome is being drafted for a stage 1 larva - because stage 1 larvae are much smaller than stage 3 larvae. However, most behaviour analyses have been performed for stage 3 larvae because their larger size makes them easier to handle and observe. It is therefore warranted to either redo the electron microscopic reconstruction for a stage 3 larva or to survey the behavioural faculties of stage 1 larvae. We provide the latter. In a community-based approach we called the Ol1mpiad, we probed stage 1 Drosophila larvae for free locomotion, feeding, responsiveness to substrate vibration, gentle and nociceptive touch, burrowing, olfactory preference and thermotaxis, light avoidance, gustatory choice of various tastants plus odour-taste associative learning, as well as light/dark-electric shock associative learning. Quantitatively, stage 1 larvae show lower scores in most tasks, arguably because of their smaller size and lower speed. Qualitatively, however, stage 1 larvae perform strikingly similar to stage 3 larvae in almost all cases. These results bolster confidence in mapping brain structure and behaviour across developmental stages.
Language
  • English
Open access status
bronze
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Persistent URL
https://sonar.ch/global/documents/2332
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