Impact of porcine cytomegalovirus on long-term orthotopic cardiac xenotransplant survival.
- Denner J Robert Koch Institute, Berlin, Germany. DennerJ@rki.de.
- Längin M Department of Anaesthesiology, University Hospital, Ludwig-Maximilians-Universität München, Munich, Germany.
- Reichart B Walter Brendel Centre of Experimental Medicine, Ludwig-Maximilians-Universität München, Munich, Germany.
- Krüger L Robert Koch Institute, Berlin, Germany.
- Fiebig U Robert Koch Institute, Berlin, Germany.
- Mokelke M Walter Brendel Centre of Experimental Medicine, Ludwig-Maximilians-Universität München, Munich, Germany.
- Radan J Walter Brendel Centre of Experimental Medicine, Ludwig-Maximilians-Universität München, Munich, Germany.
- Mayr T Department of Anaesthesiology, University Hospital, Ludwig-Maximilians-Universität München, Munich, Germany.
- Milusev A Department of Biomedical Research (DMBR), University of Bern, Bern, Switzerland.
- Luther F Department of Biomedical Research (DMBR), University of Bern, Bern, Switzerland.
- Sorvillo N Department of Biomedical Research (DMBR), University of Bern, Bern, Switzerland.
- Rieben R Department of Biomedical Research (DMBR), University of Bern, Bern, Switzerland.
- Brenner P Department of Cardiac Surgery, University Hospital, Maximilians-Universität München, Munich, Germany.
- Walz C Institute of Pathology, Faculty of Medicine, Ludwig-Maximilians-Universität München, Munich, Germany.
- Wolf E Animal Breeding and Biotechnology, Gene Center and Department of Veterinary Sciences, Ludwig-Maximilians-Universität München, Munich, Germany.
- Roshani B Unit of Infection Models, German Primate Center, Göttingen, Germany.
- Stahl-Hennig C Unit of Infection Models, German Primate Center, Göttingen, Germany.
- Abicht JM Department of Anaesthesiology, University Hospital, Ludwig-Maximilians-Universität München, Munich, Germany.
- 2020-10-17
Published in:
- Scientific reports. - 2020
English
Xenotransplantation using pig organs has achieved survival times up to 195 days in pig orthotopic heart transplantation into baboons. Here we demonstrate that in addition to an improved immunosuppressive regimen, non-ischaemic preservation with continuous perfusion and control of post-transplantation growth of the transplant, prevention of transmission of the porcine cytomegalovirus (PCMV) plays an important role in achieving long survival times. For the first time we demonstrate that PCMV transmission in orthotopic pig heart xenotransplantation was associated with a reduced survival time of the transplant and increased levels of IL-6 and TNFα were found in the transplanted baboon. Furthermore, high levels of tPA-PAI-1 complexes were found, suggesting a complete loss of the pro-fibrinolytic properties of the endothelial cells. These data show that PCMV has an important impact on transplant survival and call for elimination of PCMV from donor pigs.
- Language
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- English
- Open access status
- gold
- Identifiers
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- DOI 10.1038/s41598-020-73150-9
- PMID 33067513
- Persistent URL
- https://sonar.ch/global/documents/233763
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