Journal article
Cellogram: On-the-Fly Traction Force Microscopy.
- Lendenmann T Laboratory of Thermodynamics in Emerging Technologies and #Institute for Mechanical Systems , ETH Zurich , Zurich 8092 , Switzerland.
- Schneider T Courant Institute of Mathematical Sciences , New York University , New York 10003 , United States.
- Dumas J Courant Institute of Mathematical Sciences , New York University , New York 10003 , United States.
- Tarini M Department of Computer Science , Università degli Studi di Milano , Milano 20133 , Italy.
- Giampietro C Swiss Federal Laboratories for Materials Science and Technology , EMPA , Dübendorf 8600 , Switzerland.
- Bajpai A Department of Mechanical and Aerospace Engineering , New York University , New York 11201 , United States.
- Chen W Department of Mechanical and Aerospace Engineering , New York University , New York 11201 , United States.
- Gerber J Laboratory of Thermodynamics in Emerging Technologies and #Institute for Mechanical Systems , ETH Zurich , Zurich 8092 , Switzerland.
- Poulikakos D Laboratory of Thermodynamics in Emerging Technologies and #Institute for Mechanical Systems , ETH Zurich , Zurich 8092 , Switzerland.
- Ferrari A Laboratory of Thermodynamics in Emerging Technologies and #Institute for Mechanical Systems , ETH Zurich , Zurich 8092 , Switzerland.
- Panozzo D Courant Institute of Mathematical Sciences , New York University , New York 10003 , United States.
- 2019-09-21
Published in:
- Nano letters. - 2019
English
Traction force microscopy (TFM) derives maps of cell-generated forces, typically in the nanonewton range, transmitted to the extracellular environment upon actuation of complex biological processes. In traditional approaches, force rendering requires a terminal, time-consuming step of cell deadhesion to obtain a reference image. A conceptually opposite approach is provided by reference-free methods, opening to the on-the-fly generation of force maps from an ongoing experiment. This requires an image processing algorithm keeping the pace of the biological phenomena under investigation. Here, we introduce an integrated software pipeline rendering force maps from single reference-free TFM images seconds to minutes after their acquisition. The algorithm tackles image processing, reference image estimation, and finite element analysis as a single problem, yielding a robust and fully automatic solution. The method's capabilities are demonstrated in two applications. First, the mechanical annihilation of cancer cells is monitored as a function of rising environmental temperature, setting a population threshold at 45 °C. Second, the fast temporal correlation of forces produced across individual cells is used to map physically connected adhesion points, yielding typical lengths that vary as a function of the cell cycle phase.
- Language
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- English
- Open access status
- closed
- Identifiers
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- DOI 10.1021/acs.nanolett.9b01505
- PMID 31538794
- Persistent URL
- https://sonar.ch/global/documents/234302
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