![Unlabelled iododeoxyuridine increases the cytotoxicity and incorporation of [1251]-iododeoxyuridine in two human glioblastoma cell lines.](/static/images/no-image.png)
Journal article
Unlabelled iododeoxyuridine increases the cytotoxicity and incorporation of [1251]-iododeoxyuridine in two human glioblastoma cell lines.
- Xiao WH Division of Nuclear Medicine, University Hospital of Geneva, Switzerland.
- Dupertuis YM
- Mermillod B
- Sun LQ
- de Tribolet N
- Buchegger F
- 2000-12-29
Published in:
- Nuclear medicine communications. - 2000
Binding, Competitive
Cell Survival
DNA, Neoplasm
Glioblastoma
Humans
Idoxuridine
Iodine Radioisotopes
Radiopharmaceuticals
Tumor Cells, Cultured
English
Iododeoxyuridine (IUdR), labelled with radioiodines emitting Auger, alpha or beta- radiation, has been proposed as a therapeutic tool in the treatment of cancer. However, the low per cent incorporation in tumour cells and limited cytotoxicity are major obstacles for such an application. Using unlabelled IUdR as a modulator, we have studied the in vitro cytotoxicity of [125I]-IUdR in two human glioblastoma cell lines. Surprisingly, an enhanced cytotoxicity of [125I]-IUdR was observed in the presence of 0.3-10 microM concentrations of unlabelled IUdR in U251 glioblastoma cells and to a lesser extent in LN229 cells. The presence of unlabelled IUdR unexpectedly increased the incorporation of [125I]-IUdR in both cell lines. Thymidine competitively blocked the cytotoxic effects of combined unlabelled and [125I]-labelled IUdR in these cells and DNA-incorporation of radiolabelled IUdR.
- Language
-
- English
- Open access status
- closed
- Identifiers
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- DOI 10.1097/00006231-200010000-00009
- PMID 11130336
- Persistent URL
- https://sonar.ch/global/documents/236804
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