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Comparative genetic analysis of tritrichomonadid protozoa by the random amplified polymorphic DNA technique.
Journal article

Comparative genetic analysis of tritrichomonadid protozoa by the random amplified polymorphic DNA technique.

  • Felleisen RS Institute of Parasitology, University of Berne, Switzerland. felleisen@ipa.unibe.ch
  • 1998-03-11
Published in:
  • Parasitology research. - 1998
Animals
Cattle
DNA Fingerprinting
DNA, Protozoan
Random Amplified Polymorphic DNA Technique
Saimiri
Species Specificity
Swine
Tritrichomonas
English The taxonomic classification within the genus Tritrichomonas is a subject of controversy, and, potentially, separation of the tritrichomonads from cattle and swine on the species level is not valid. To tackle this hypothesis we comparatively assessed several isolates of protozoan parasites from the three Tritrichomonas species T. foetus, T. suis, and T. mobilensis by the RAPD (random amplified polymorphic DNA) technique. In this method with 20 different primers, all T. foetus and T. suis isolates resulted in identical genomic fingerprints, thus yielding additional evidence for the genetic identity of T. foetus and T. suis. In contrast, it turned out that the species T. mobilensis isolated from the squirrel monkey is genetically distinct and can clearly be discriminated from the other tritrichomonads. Consequently, the results obtained in this study support a possible future revision of the taxonomic classification of the genus Tritrichomonas.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/237437
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