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Cooperative interactions of AP-1 and basic helix-loop-helix transcription factors regulate T1 gene expression.
Journal article

Cooperative interactions of AP-1 and basic helix-loop-helix transcription factors regulate T1 gene expression.

  • 1997-07-01
Published in:
  • Biological chemistry. - 1997
3T3 Cells
Animals
Base Sequence
Basic Helix-Loop-Helix Transcription Factors
Cell Line
DNA-Binding Proteins
Enhancer Elements, Genetic
G1 Phase
Gene Expression Regulation
Helix-Loop-Helix Motifs
Interleukin-1 Receptor-Like 1 Protein
Membrane Proteins
Mice
Molecular Sequence Data
Promoter Regions, Genetic
Protein Binding
Proteins
Receptors, Interleukin
Recombinant Fusion Proteins
Resting Phase, Cell Cycle
Transcription Factor AP-1
Transcription Factors
Tumor Cells, Cultured
English The T1 gene is a murine, delayed early serum-responsive gene that encodes glycoproteins of the interleukin-1 receptor (IL-1R) family. Transcription of the T1 gene leads to production of two mRNAs that encode a transmembrane protein, which is highly similar to the type-1 IL-1R, and a secreted protein, which consists solely of the extracellular part. Fibroblasts, in contrast to mast cells, express predominantly the shorter form of the protein, and several mitogens cause strong, transient induction of the T1 gene in these cells. Here we describe the identification of a 148 bp enhancer element that is positioned 3.6 kb upstream of the transcription initiation site. A TPA-responsive element (TRE) and three identical E-boxes are located within this sequence. Introduced point mutations confirmed the necessity of these sites for full T1 promoter activity. The TRE and the distal E-box are absolutely indispensable for promoter function, whereas the two proximal E-boxes contribute less to promoter strength. In vitro the three E-boxes are bound by different protein complexes.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/237814
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