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Isolation of 8-methoxypsoralen accessible DNA domains from chromatin of intact cells.
Journal article

Isolation of 8-methoxypsoralen accessible DNA domains from chromatin of intact cells.

  • Mathis GA University of Zürich-Tierspital, Institute of Pharmacology and Biochemistry, Switzerland.
  • Althaus FR
  • 1990-01-01
Published in:
  • Cell biology and toxicology. - 1990
Animals
Cells, Cultured
Chromatin
DNA
Deoxyribonucleases, Type I Site-Specific
Electrophoresis, Polyacrylamide Gel
Hydrolysis
Liver
Male
Methoxsalen
Nucleic Acid Denaturation
Nucleic Acid Renaturation
Rats
English The chromatin organization of living mammalian cells was probed using 8-methoxypsoralen (MOP). In intact cells, MOP intercalates into DNA domains which are also preferentially accessible to micrococcal nuclease. After UV365 nm irradiation of MOP-treated cells, this chemical forms bifunctional adducts crosslinking the two strands of DNA. Following extraction of cellular DNA, heat denaturation and renaturation at low temperature, the fraction of crosslinked DNA is obtained following enzymatic hydrolysis of unhybridized, non-crosslinked DNA by nuclease S1 treatment. An application of this procedure in the isolation of 8-methoxypsoralen-accessible DNA domains during DNA excision repair is shown.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/237907
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