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Plasma membrane poration induced by ultrasound exposure: implication for drug delivery.
Journal article

Plasma membrane poration induced by ultrasound exposure: implication for drug delivery.

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  • 2005-05-04
Published in:
  • Journal of controlled release : official journal of the Controlled Release Society. - 2005
Animals
Cell Line, Tumor
Cell Membrane
Cell Membrane Permeability
Drug Delivery Systems
Microscopy, Confocal
Porosity
Rats
Ultrasonics
English Sonoporation, in the presence of ultrasound contrast agents (UCA), is a technique that permits the transfer of drugs, including genes, into cells. In this study, the size of the pores created by ultrasound application, and the duration of pore opening, have been characterized via indirect molecular probing and microscopic observation. Internalization of molecules with diameters up to 37 nm was efficient and generally well-tolerated; on the other hand, confocal microscopy revealed that 75 nm particles entered only a few cells when sonoporation was applied. In general, the larger the species to internalize, the poorer the transfer. Direct visualization of pores following insonification, using scanning electron microscopy, was hampered by the presence of numerous villi on the surface of the cells employed (MAT B III), and by the short duration of pore opening. Clearer observations of porated regions were possible using red blood cells. This research (i) confirms that sonoporation is a means with which to achieve macromolecule delivery into cells, and (ii) characterizes in some detail the phenomenon of ultrasound induction of transient pores in the cell membrane.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/239890
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