Multimodal X-ray imaging of nanocontainer-treated macrophages and calcium distribution in the perilacunar bone matrix.
- Stachnik K DESY Photon Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, 22607, Germany. karolina.stachnik@desy.de.
- Warmer M DESY Photon Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, 22607, Germany.
- Mohacsi I Center for Free-Electron Laser Science, Hamburg, 22607, Germany.
- Hennicke V DESY Photon Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, 22607, Germany.
- Fischer P DESY Photon Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, 22607, Germany.
- Meyer J DESY Photon Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, 22607, Germany.
- Spitzbart T DESY Photon Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, 22607, Germany.
- Barthelmess M DESY Photon Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, 22607, Germany.
- Eich J Research Center Borstel - Leibniz Lung Center, Borstel, 23845, Germany.
- David C Paul Scherrer Institute, Villigen, PSI, 5232, Switzerland.
- Feldmann C Institute of Inorganic Chemistry, Karlsruhe Institute of Technology (KIT), Karlsruhe, 76131, Germany.
- Busse B Department of Osteology and Biomechanics, University Medical Center Hamburg-Eppendorf, Hamburg, 22529, Germany.
- Jähn K Department of Osteology and Biomechanics, University Medical Center Hamburg-Eppendorf, Hamburg, 22529, Germany.
- Schaible UE Research Center Borstel - Leibniz Lung Center, Borstel, 23845, Germany.
- Meents A DESY Photon Science, Deutsches Elektronen-Synchrotron DESY, Hamburg, 22607, Germany.
- 2020-02-06
Published in:
- Scientific reports. - 2020
English
Studies of biological systems typically require the application of several complementary methods able to yield statistically-relevant results at a unique level of sensitivity. Combined X-ray fluorescence and ptychography offer excellent elemental and structural imaging contrasts at the nanoscale. They enable a robust correlation of elemental distributions with respect to the cellular morphology. Here we extend the applicability of the two modalities to higher X-ray excitation energies, permitting iron mapping. Using a long-range scanning setup, we applied the method to two vital biomedical cases. We quantified the iron distributions in a population of macrophages treated with Mycobacterium-tuberculosis-targeting iron-oxide nanocontainers. Our work allowed to visualize the internalization of the nanocontainer agglomerates in the cytosol. From the iron areal mass maps, we obtained a distribution of antibiotic load per agglomerate and an average areal concentration of nanocontainers in the agglomerates. In the second application we mapped the calcium content in a human bone matrix in close proximity to osteocyte lacunae (perilacunar matrix). A concurrently acquired ptychographic image was used to remove the mass-thickness effect from the raw calcium map. The resulting ptychography-enhanced calcium distribution allowed then to observe a locally lower degree of mineralization of the perilacunar matrix.
- Language
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- English
- Open access status
- gold
- Identifiers
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- DOI 10.1038/s41598-020-58318-7
- PMID 32019946
- Persistent URL
- https://sonar.ch/global/documents/240343
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