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Intermittent fasting improves metabolic flexibility in short-term high-fat diet-fed mice.
Journal article

Intermittent fasting improves metabolic flexibility in short-term high-fat diet-fed mice.

  • Dedual MA Division of Pediatric Endocrinology and Diabetology, University Children's Hospital, Zurich, Switzerland.
  • Wueest S Division of Pediatric Endocrinology and Diabetology, University Children's Hospital, Zurich, Switzerland.
  • Borsigova M Division of Pediatric Endocrinology and Diabetology, University Children's Hospital, Zurich, Switzerland.
  • Konrad D Division of Pediatric Endocrinology and Diabetology, University Children's Hospital, Zurich, Switzerland.
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  • 2019-09-11
Published in:
  • American journal of physiology. Endocrinology and metabolism. - 2019
glucose intolerance
high-fat diet
insulin resistance
metabolic flexibility
obesity
Adipose Tissue
Animals
Body Weight
Diet, High-Fat
Eating
Fasting
Fatty Acids, Nonesterified
Glucose Tolerance Test
Insulin Resistance
Lipid Metabolism
Liver
Male
Mice
Mice, Inbred C57BL
Pulmonary Gas Exchange
Triglycerides
English Four days of high-fat diet (HFD) feeding are sufficient to induce glucose intolerance and hepatic steatosis in mice. While prolonged HFD-induced metabolic complications are partly mediated by increased food intake during the light (inactive) phase, such a link has not yet been established in short-term HFD-fed mice. Herein, we hypothesized that a short bout of HFD desynchronizes feeding behavior, thereby contributing to glucose intolerance and hepatic steatosis. To this end, 12-wk-old C57BL/6J littermates were fed a HFD for 4 days either ad libitum or intermittently. Intermittent-fed mice were fasted for 8 h during their inactive phase. Initiation of HFD led to an immediate increase in food intake already during the first light phase. Moreover, glucose tolerance was significantly impaired in ad libitum- but not in intermittent HFD-fed mice, indicating that desynchronized feeding behavior contributes to short-term HFD-induced glucose intolerance. Of note, overall food intake was similar between the groups, as was body weight. However, intermittent HFD-fed mice revealed higher fat depot weights. Phosphorylation of hormone sensitivity lipase and free fatty acid release from isolated adipocytes were significantly elevated, suggesting increased lipolysis in intermittent HFD-fed mice. Moreover, hepatic mRNA expression of lipogenetic enzymes and liver triglyceride levels were significantly increased in intermittent HFD-fed mice. Importantly, food deprivation decreased respiratory exchange ratio promptly in intermittent- but not in ad libitum HFD-fed mice. In conclusion, retaining a normal feeding pattern prevented HFD-induced impairment of metabolic flexibility in short-term HFD-fed mice.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/241111
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