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A rational and iterative process for targeted nanoparticle design and validation.

  • Rodriguez-Lorenzo L Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland. Electronic address: laura.rodriguez-lorenzo@inl.int.
  • Rafiee SD Deparment of Oncology, Microbiology and Immunology, Faculty of Sciences and Medicine, University of Fribourg, Chemin du Musée 18, 1700 Fribourg, Switzerland.
  • Reis C Deparment of Oncology, Microbiology and Immunology, Faculty of Sciences and Medicine, University of Fribourg, Chemin du Musée 18, 1700 Fribourg, Switzerland.
  • Milosevic A Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland.
  • Moore TL Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland.
  • Balog S Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland.
  • Rothen-Rutishauser B Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland.
  • Ruegg C Deparment of Oncology, Microbiology and Immunology, Faculty of Sciences and Medicine, University of Fribourg, Chemin du Musée 18, 1700 Fribourg, Switzerland.
  • Petri-Fink A Adolphe Merkle Institute, University of Fribourg, Chemin des Verdiers 4, 1700 Fribourg, Switzerland; Chemistry Department, Faculty of Sciences and Medicine, University of Fribourg, Chemin du Musée 9, 1700 Fribourg, Switzerland. Electronic address: alke.fink@unifr.ch.
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  • 2018-08-12
Published in:
  • Colloids and surfaces. B, Biointerfaces. - 2018
Anti-biofouling
DF-HIS
ERBB2-positive breast cancer cells
FACS
ICP-OES
Multifunctional gold nanoparticles
Trastuzumab
Breast Neoplasms
Cell Line, Tumor
Female
Gold
Humans
Metal Nanoparticles
Particle Size
Receptor, ErbB-2
Surface Properties
English The lack of understanding of fundamental nano-bio interactions, and difficulties in designing particles stable in complex biological environments are major limitations to their translation into biomedical clinical applications. Here we present a multi-parametric approach to fully characterize targeted nanoparticles, and emphasizes the significant effect that each detail in the synthetic process can have on downstream in vitro results. Through an iterative process, particles were designed, synthesized and tested for physico-chemical and bio-interactive properties which allowed the optimization of nanoparticle functionality. Taken together all interative steps demonstrate that we have synthesized a multifunctional gold nanoparticles that can detect ERBB2-positive breast cancer cells while showing stealth-like behavior toward ERBB2-negative cells and excellent physicochemical stability.
Language
  • English
Open access status
green
Identifiers
Persistent URL
https://sonar.ch/global/documents/2423
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