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Development of a multiplex real-time quantitative PCR assay to detect Chlamydia pneumoniae, Legionella pneumophila and Mycoplasma pneumoniae in respiratory tract secretions.
Journal article

Development of a multiplex real-time quantitative PCR assay to detect Chlamydia pneumoniae, Legionella pneumophila and Mycoplasma pneumoniae in respiratory tract secretions.

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  • 2003-03-05
Published in:
  • Diagnostic microbiology and infectious disease. - 2003
Biotechnology
Chlamydophila Infections
Chlamydophila pneumoniae
DNA, Bacterial
Humans
Legionella pneumophila
Legionnaires' Disease
Mycoplasma pneumoniae
Pneumonia, Bacterial
Pneumonia, Mycoplasma
Polymerase Chain Reaction
Respiratory System
Retrospective Studies
Sensitivity and Specificity
English Atypical pathogens such as Chlamydia pneumoniae, Legionella pneumophila and Mycoplasma pneumoniae are an important cause of community-acquired pneumonia. The available detection methods (culture and serology) either lack sensitivity or give only a retrospective diagnosis. In order to improve their detection and quantification in respiratory samples, a real-time multiplex PCR, performed in two separate reactions, was developed for these three pathogens. The comparison of multiplex real-time and conventional PCR assay on 73 respiratory specimens showed an overall agreement of 98.3%, corresponding to 95.8%, 100% and 100% agreement for C. pneumoniae, L. pneumophila and M. pneumoniae, respectively. Clinical application of this multiplex real-time PCR was done on 40 respiratory samples from 38 patients with respiratory tract infections. Of 19 serology-positive patients, 14 were confirmed by the multiplex real-time PCR to be infected by either one of the three pathogens. All samples from serology-negative patients were negative with the multiplex real-time PCR.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/242488
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