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Anesthetic pretreatment confers thermotolerance on Saccharomyces cerevisiae yeast.
Journal article

Anesthetic pretreatment confers thermotolerance on Saccharomyces cerevisiae yeast.

  • Luethy A Department of Anesthesia, Critical Care, and Pain Medicine, Massachusetts General Hospital, 55 Fruit Street, Boston, MA, 02114, USA; Department of Anesthesia, Kantonsspital Aarau, Tellstrasse 25, 5001, Aarau, Switzerland. Electronic address: anita.luethy@ksa.ch.
  • Kindler CH Department of Anesthesia, Kantonsspital Aarau, Tellstrasse 25, 5001, Aarau, Switzerland. Electronic address: christoph.kindler@ksa.ch.
  • Cotten JF Department of Anesthesia, Critical Care, and Pain Medicine, Massachusetts General Hospital, 55 Fruit Street, Boston, MA, 02114, USA. Electronic address: jcotten@mgh.harvard.edu.
  • 2019-11-30
Published in:
  • Biochemical and biophysical research communications. - 2020
Alcohol
Anesthetic
Heat shock
Preconditioning
Stress response
Yeast
Amino Acids
Anesthetics
Ethanol
Saccharomyces cerevisiae
Temperature
Thermotolerance
English Saccharomyces cerevisiae yeast, when pretreated with elevated temperatures, undergo adaptive changes that promote survival after an otherwise lethal heat stress. The heat shock response, a cellular stress response variant, mediates these adaptive changes. Ethanol, a low-potency anesthetic, promotes thermotolerance possibly through heat shock response activation. Therefore, we hypothesized other anesthetic compounds, like ethanol, may invoke the heat shock response to promote thermotolerance. To test this hypothesis, we pretreated yeast with a series of non-volatile anesthetic and anesthetic-related compounds and quantified survival following lethal heat shock (52 °C for 5 min). Most compounds invoked thermoprotection and promoted survival with a potency proportional to hydrophobicity: tribromoethanol (5.6 mM, peak survival response), trichloroethanol (17.8 mM), dichloroethanol (100 mM), monochloroethanol (316 mM), trifluoroethanol (177.8 mM), ethanol (1 M), isopropanol (1 M), propofol (316 μM), and carbon tetrabromide (32 μM). Thermoprotection conferred by pretreatment with elevated temperatures was "left shifted" by anesthetic co-treatment from (in °C) 35.3 ± 0.1 to 32.2 ± 0.1 with trifluoroethanol (177.8 mM), to 31.2 ± 0.1 with trichloroethanol (17.8 mM), and to 29.1 ± 0.3 with tribromoethanol (5.6 mM). Yeast in postdiauxic shift growth phase, relative to mid-log, responded with greater heat shock survival; and media supplementation with tryptophan and leucine blocked thermoprotection, perhaps by reversing the amino acid starvation response. Our results suggest S. cerevisase may serve as a model organism for understanding anesthetic toxicity and anesthetic preconditioning, a process by which anesthetics promote tissue survival after hypoxic insult.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/24586
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