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Susceptibility testing of Mycobacterium abscessus by isothermal microcalorimetry.
Journal article

Susceptibility testing of Mycobacterium abscessus by isothermal microcalorimetry.

  • Boillat-Blanco N Infectious Diseases Service, Department of Medicine, University Hospital and University of Lausanne, Lausanne, Switzerland. Electronic address: noemie.boillat@chuv.ch.
  • Furustrand Tafin U Infectious Diseases Service, Department of Medicine, University Hospital and University of Lausanne, Lausanne, Switzerland; Septic Surgical Unit, Department of Surgery, University Hospital and University of Lausanne, Lausanne, Switzerland. Electronic address: hanna.furustrand@chuv.ch.
  • Jaton K Institute of Microbiology, University Hospital and University of Lausanne, Lausanne, Switzerland. Electronic address: katia.jaton-ogay@chuv.ch.
  • Trampuz A Center for Musculoskeletal Surgery, Charité - University Medicine, Free and Humboldt - University of Berlin, Berlin, Germany. Electronic address: andrej.trampuz@charite.de.
  • 2015-07-27
Published in:
  • Diagnostic microbiology and infectious disease. - 2015
Antibiotics
Microcalorimetry
Mycobacteria
Mycobacterium abscessus
Susceptibility testing
Anti-Bacterial Agents
Calorimetry
Humans
Microbial Sensitivity Tests
Mycobacterium
Mycobacterium Infections, Nontuberculous
Temperature
Time Factors
English We evaluated a new method for susceptibility testing of a rapidly growing mycobacterium using real-time measurement of heat (microcalorimetry). MICs of 2 clinical Mycobacterium abscessus isolates were determined by microbroth dilution and E-test. For microcalorimetry, Middlebrook-7H10 agar+10% oleic acid-albumin-dextrose-catalase, containing amikacin, clarithromycin, linezolid, and ciprofloxacin was inoculated with ~10(5)CFU/mL. Heat production was measured at 37°C for 72h. Minimal heat inhibition concentration (MHIC) was defined as the lowest antibiotic concentration inhibiting growth-related heat production. Growth of M. abscessus was detected after a median of 16.5h (range, 8.5-26.9h). Heat detection was proportionally delayed with increasing concentration of antibiotics. MHICs for the tested strains were 16 to >16mg/L for amikacin, >8mg/L for clarithromycin, 4 to >16mg/L for ciprofloxacin, 24 to >32mg/L for linezolid. MHICs were in agreement within two 2-fold dilutions with conventional MICs. Microcalorimetry may accelerate antimicrobial susceptibility testing in mycobacteria and provide additional real-time information on the drug effect.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/246218
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