High capacity in G protein-coupled receptor signaling.
- Keshelava A Department of Pharmacology and Toxicology, Faculty of Biology and Medicine, University of Lausanne, 1011, Lausanne, Switzerland.
- Solis GP Department of Pharmacology and Toxicology, Faculty of Biology and Medicine, University of Lausanne, 1011, Lausanne, Switzerland.
- Hersch M Department of Computational Biology, Faculty of Biology and Medicine, University of Lausanne, 1011, Lausanne, Switzerland.
- Koval A Department of Pharmacology and Toxicology, Faculty of Biology and Medicine, University of Lausanne, 1011, Lausanne, Switzerland.
- Kryuchkov M Department of Pharmacology and Toxicology, Faculty of Biology and Medicine, University of Lausanne, 1011, Lausanne, Switzerland.
- Bergmann S Department of Computational Biology, Faculty of Biology and Medicine, University of Lausanne, 1011, Lausanne, Switzerland. sven.bergmann@unil.ch.
- Katanaev VL Department of Pharmacology and Toxicology, Faculty of Biology and Medicine, University of Lausanne, 1011, Lausanne, Switzerland. vladimir.katanaev@unil.ch.
- 2018-03-02
Published in:
- Nature communications. - 2018
Acetylcholine
Calcium
Cell Line
Cell Membrane
HEK293 Cells
Humans
Muscarinic Agonists
Receptor, Muscarinic M3
Receptors, G-Protein-Coupled
Signal Transduction
English
G protein-coupled receptors (GPCRs) constitute a large family of receptors that activate intracellular signaling pathways upon detecting specific extracellular ligands. While many aspects of GPCR signaling have been uncovered through decades of studies, some fundamental properties, like its channel capacity-a measure of how much information a given transmission system can reliably transduce-are still debated. Previous studies concluded that GPCRs in individual cells could transmit around one bit of information about the concentration of the ligands, allowing only for a reliable on or off response. Using muscarinic receptor-induced calcium response measured in individual cells upon repeated stimulation, we show that GPCR signaling systems possess a significantly higher capacity. We estimate the channel capacity of this system to be above two, implying that at least four concentration levels of the agonist can be distinguished reliably. These findings shed light on the basic principles of GPCR signaling.
- Language
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- English
- Open access status
- gold
- Identifiers
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- DOI 10.1038/s41467-018-02868-y
- PMID 29491460
- Persistent URL
- https://sonar.ch/global/documents/246568
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