Generation and characterization of dromedary Tenascin-C and Tenascin-W specific antibodies.

Dhaouadi S; Murdamoothoo D; Tounsi A; Erne W; Benabderrazek R; Benlasfar Z; Hendaoui L; Chiquet-Ehrismann R; Boubaker S; Orend G; Hendaoui I; Bouhaouala-Zahar B

doi:10.1016/j.bbrc.2020.05.077

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Journal article

Generation and characterization of dromedary Tenascin-C and Tenascin-W specific antibodies.

Dhaouadi S Laboratoire des Venins et Molécules Thérapeutiques, Institut Pasteur Tunis, 13 Place Pasteur, BP74, 1002, Tunis- Université Tunis El Manar Tunisia, Tunisia.
Murdamoothoo D INSERM U1109, Tumor Microenvironment Group, 1 Place de L'Hôpital, 67091, Strasbourg, France.
Tounsi A Laboratoire des Venins et Molécules Thérapeutiques, Institut Pasteur Tunis, 13 Place Pasteur, BP74, 1002, Tunis- Université Tunis El Manar Tunisia, Tunisia.
Erne W INSERM U1109, Tumor Microenvironment Group, 1 Place de L'Hôpital, 67091, Strasbourg, France.
Benabderrazek R Laboratoire des Venins et Molécules Thérapeutiques, Institut Pasteur Tunis, 13 Place Pasteur, BP74, 1002, Tunis- Université Tunis El Manar Tunisia, Tunisia.
Benlasfar Z Laboratoire des Venins et Molécules Thérapeutiques, Institut Pasteur Tunis, 13 Place Pasteur, BP74, 1002, Tunis- Université Tunis El Manar Tunisia, Tunisia.
Hendaoui L Service de Radiologie, Hôpital Mongi Slim-La Marsa-Tunisia, Tunisia.
Chiquet-Ehrismann R Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, Basel, Switzerland.
Boubaker S Service Anatomopathologie, Institut Pasteur Tunis, 13 Place Pasteur, BP74, 1002, Tunis- Université Tunis El Manar Tunisia, Tunisia.
Orend G INSERM U1109, Tumor Microenvironment Group, 1 Place de L'Hôpital, 67091, Strasbourg, France.
Hendaoui I Friedrich Miescher Institute for Biomedical Research, Maulbeerstrasse 66, Basel, Switzerland.
Bouhaouala-Zahar B Laboratoire des Venins et Molécules Thérapeutiques, Institut Pasteur Tunis, 13 Place Pasteur, BP74, 1002, Tunis- Université Tunis El Manar Tunisia, Tunisia; Faculté de Médecine de Tunis, 15 Rue Djebel Lakhdhar, La Rabta, 1007, Tunis-Université Tunis el Manar, Tunisia. Electronic address: balkiss.bouhaouala@fmt.utm.tn.

2020-06-29

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Biochemical and biophysical research communications. - 2020

English Tenascin-C (TNC) and tenascin-W (TNW), large hexameric glycoproteins overexpressed in the tumor microenvironment, are useful tumor biomarkers for theranostic applications. For now, polyclonal and monoclonal antibodies, as well as aptamers targeting TNC and TNW have been developed. However, the immunostaining sensitivity of antibodies is very heterogenous. The main aim of this study was to generate antibodies in dromedary that detect TNC and TNW, respectively. We show that immune sera from immunized dromedaries are able to specifically bind native TNC and TNW by ELISA and also to detect TNC and TNW in matrix tracks of mammary tumors by immunostaining. Furthermore, we demonstrate that purified IgG subtypes are able to interact specifically with TNC or TNW by ELISA and immunostaining. These camelid antibodies are a good basis to develop tools for the detection of TNC and TNW in the tumor microenvironment and could potentially have a broader application for early diagnosis of solid cancers.

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