Drs2p-related P-type ATPases Dnf1p and Dnf2p Are Required for Phospholipid Translocation across the Yeast Plasma Membrane and Serve a Role in Endocytosis
- Pomorski, Thomas Department of Cell Biology and Histology, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands;
- Lombardi, Ruben Department of Biochemistry, University of Geneva, Geneva CH-1211, Switzerland; and
- Riezman, Howard Department of Biochemistry, University of Geneva, Geneva CH-1211, Switzerland; and
- Devaux, Philippe F. Institut de Biologie Physico-chimique, 75005 Paris, France
- van Meer, Gerrit Department of Membrane Enzymology, Center for Biomembranes and Lipid Enzymology, Utrecht University, 3584 CH Utrecht, The Netherlands;
- Holthuis, Joost C. M. Department of Cell Biology and Histology, Academic Medical Center, 1105 AZ Amsterdam, The Netherlands;
Published in:
- Molecular Biology of the Cell. - American Society for Cell Biology (ASCB). - 2003, vol. 14, no. 3, p. 1240-1254
English
Plasma membranes in eukaryotic cells display asymmetric lipid distributions with aminophospholipids concentrated in the inner and sphingolipids in the outer leaflet. This asymmetry is maintained by ATP-driven lipid transporters whose identities are unknown. The yeast plasma membrane contains two P-type ATPases, Dnf1p and Dnf2p, with structural similarity to ATPase II, a candidate aminophospholipid translocase from bovine chromaffin granules. Loss of Dnf1p and Dnf2p virtually abolished ATP-dependent transport of NBD-labeled phosphatidylethanolamine, phosphatidylserine, and phosphatidylcholine from the outer to the inner plasma membrane leaflet, leaving transport of sphingolipid analogs unaffected. Labeling with trinitrobenzene sulfonic acid revealed that the amount of phosphatidylethanolamine exposed on the surface of Δdnf1Δdnf2 cells increased twofold relative to wild-type cells. Phosphatidylethanolamine exposure by Δdnf1Δdnf2 cells further increased upon removal of Drs2p, an ATPase II homolog in the yeast Golgi. These changes in lipid topology were accompanied by a cold-sensitive defect in the uptake of markers for bulk-phase and receptor-mediated endocytosis. Our findings demonstrate a requirement for Dnf1p and Dnf2p in lipid translocation across the yeast plasma membrane. Moreover, it appears that Dnf1p, Dnf2p and Drs2p each help regulate the transbilayer lipid arrangement in the plasma membrane, and that this regulation is critical for budding endocytic vesicles.
- Language
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- English
- Open access status
- green
- Identifiers
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- DOI 10.1091/mbc.e02-08-0501
- ISSN 1059-1524
- Persistent URL
- https://sonar.ch/global/documents/247713
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