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Isolation and Validation of Anti-B7-H4 scFvs from an Ovarian Cancer scFv Yeast-Display Library.
Journal article

Isolation and Validation of Anti-B7-H4 scFvs from an Ovarian Cancer scFv Yeast-Display Library.

  • Dangaj D Department of Oncology, Ludwig Cancer Research Center, University of Lausanne, Lausanne, Switzerland.
  • Scholler N
  • 2015-06-11
Published in:
  • Methods in molecular biology (Clifton, N.J.). - 2015
Animals
Antigen Presentation
B-Lymphocytes
Female
Gene Library
Humans
Mice
Ovarian Neoplasms
Saccharomyces cerevisiae
Single-Chain Antibodies
T-Lymphocytes
V-Set Domain-Containing T-Cell Activation Inhibitor 1
Xenograft Model Antitumor Assays
English B7-H4 (VTCN1, B7x, B7s) is an inhibitory modulator of T-cell response implicated in antigen tolerization. As such, B7-H4 is an immune checkpoint of potential therapeutic interest. To generate anti-B7-H4 targeting reagents, we isolated antibodies by differential cell screening of a yeast-display library of recombinant antibodies (scFvs) derived from ovarian cancer patients and we screened for functional scFvs capable to interfere with B7-H4-mediated inhibition of antitumor responses. We found one antibody binding to B7-H4 that could restore antitumor T cell responses. This chapter gives an overview of the methods we developed to isolate a functional anti-B7-H4 antibody fragment.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/248483
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