Journal article
Purification and amino acid sequencing of NAF, a novel neutrophil-activating factor produced by monocytes.
- Walz A Theodor-Kocher-Institut, University of Bern, Switzerland.
- Peveri P
- Aschauer H
- Baggiolini M
- 1987-12-16
Published in:
- Biochemical and biophysical research communications. - 1987
Amino Acid Sequence
Chromatography, Gel
Chromatography, High Pressure Liquid
Humans
Interleukin-8
Molecular Sequence Data
Monocytes
Neutrophils
Peptides
English
Human blood mononuclear cells were cultured for 24 h in the presence of LPS (100 ng per 5 x 10(6) cells), and a monocyte-derived neutrophil-activating factor (NAF) was purified to apparent homogeneity from the conditioned media. The purification consisted of ammonium sulphate precipitation, gel filtration, chromatography on phosphocellulose followed by hydroxylapatite, and reversed-phase HPLC on C4 and CN-propyl columns. Amino acid sequence analysis (32 of 50 presumed residues) shows that NAF is a novel peptide with little homology to known ones. Crude and pure NAF stimulated human neutrophils to release granule enzymes and to produce superoxide and H2O2.
- Language
-
- English
- Open access status
- closed
- Identifiers
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- DOI 10.1016/0006-291x(87)90432-3
- PMID 3322281
- Persistent URL
- https://sonar.ch/global/documents/249267
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