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Isolation and characterization of Kingella negevensis sp. nov., a novel Kingella species detected in a healthy paediatric population.
Journal article

Isolation and characterization of Kingella negevensis sp. nov., a novel Kingella species detected in a healthy paediatric population.

  • El Houmami N Aix-Marseille University, UM63, CNRS 7278, IRD 198, Inserm 1095, URMITE, Institut Hospitalo-Universitaire Méditerranée Infection, Marseille, France.
  • Bakour S Aix-Marseille University, UM63, CNRS 7278, IRD 198, Inserm 1095, URMITE, Institut Hospitalo-Universitaire Méditerranée Infection, Marseille, France.
  • Bzdrenga J Aix-Marseille University, UM63, CNRS 7278, IRD 198, Inserm 1095, URMITE, Institut Hospitalo-Universitaire Méditerranée Infection, Marseille, France.
  • Rathored J Aix-Marseille University, UM63, CNRS 7278, IRD 198, Inserm 1095, URMITE, Institut Hospitalo-Universitaire Méditerranée Infection, Marseille, France.
  • Seligmann H Aix-Marseille University, UM63, CNRS 7278, IRD 198, Inserm 1095, URMITE, Institut Hospitalo-Universitaire Méditerranée Infection, Marseille, France.
  • Robert C Aix-Marseille University, UM63, CNRS 7278, IRD 198, Inserm 1095, URMITE, Institut Hospitalo-Universitaire Méditerranée Infection, Marseille, France.
  • Armstrong N Aix-Marseille University, UM63, CNRS 7278, IRD 198, Inserm 1095, URMITE, Institut Hospitalo-Universitaire Méditerranée Infection, Marseille, France.
  • Schrenzel J Hôpitaux Universitaires de Genève, Service of Infectious Diseases and Service of Laboratory Medicine, Geneva, Switzerland.
  • Raoult D Aix-Marseille University, UM63, CNRS 7278, IRD 198, Inserm 1095, URMITE, Institut Hospitalo-Universitaire Méditerranée Infection, Marseille, France.
  • Yagupsky P Clinical Microbiology Laboratory, Soroka University Medical Center, Beer-Sheva, Israel.
  • Fournier PE Aix-Marseille University, UM63, CNRS 7278, IRD 198, Inserm 1095, URMITE, Institut Hospitalo-Universitaire Méditerranée Infection, Marseille, France.
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  • 2017-07-13
Published in:
  • International journal of systematic and evolutionary microbiology. - 2017
Bacterial Typing Techniques
Base Composition
Child
DNA, Bacterial
Fatty Acids
Genes, Bacterial
Humans
Israel
Kingella
Nucleic Acid Hybridization
Oropharynx
Phylogeny
RNA, Ribosomal, 16S
Sequence Analysis, DNA
Switzerland
English We herein report the isolation and characterization of 21 Gram-stain-negative strains cultivated from the oropharynx of healthy children in Israel and Switzerland. Initially described as small colony variants of Kingella kingae, phenotypic analysis, biochemical analysis, phylogenetic analysis based on sequencing of the partial 16S rRNA gene and five housekeeping genes (abcZ, adk, G6PD, groEL and recA), and whole genome sequencing and comparison between members of the genera Kingella and Neisseria provided evidence for assigning them to the genus Kingella. Cellular fatty acids included important amounts of C12 : 0, C14 : 0, C16 : 0 and C16 : 1n7. Digital DNA-DNA hybridization between the isolates Sch538T and K. kingae ATCC 23330T revealed relatedness of 19.9 %. Comparative analysis of 16S rRNA gene sequences available in GenBank allowed matches to strains isolated in the USA, suggesting a wider geographical distribution. A novel species named Kingella negevensis sp. nov. is proposed, as most strains have been isolated in the Negev, a desert region of southern Israel. The type strain is Sch538T (=CCUG 69806T=CSUR P957).
Language
  • English
Open access status
bronze
Identifiers
Persistent URL
https://sonar.ch/global/documents/252123
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