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In Vivo Measurement of Glucose Utilization in Rats using a β-Microprobe: Direct Comparison with Autoradiography

  • Millet, Philippe Unité de Neuroimagerie, Hôpitaux Universitaires de Genève;, Switzerland
  • Sallanon, Marcelle Moulin INSERM, IFR19, Institut Fédérative des Neurosciences de Lyon, Bron, France
  • Petit, Jean-Marie Laboratoire de Neuroénergétique, Université de Genève, Switzerland
  • Charnay, Yves Unité de Morphologie, Service de Neuropsychiatrie, Hôpitaux Universitaires de Genève;, Switzerland
  • Vallet, Philippe Unité de Morphologie, Service de Neuropsychiatrie, Hôpitaux Universitaires de Genève;, Switzerland
  • Morel, Christian Institute for High Energy Physics, University of Lausanne, Switzerland
  • Cespuglio, Raymond EA 3734, Université Claude Bernard Lyon, France
  • Magistretti, Pierre J. Département de Physiologie de Lausanne, Switzerland
  • Ibáñez, Vicente Unité de Neuroimagerie, Hôpitaux Universitaires de Genève;, Switzerland
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  • 2016-11-20
Published in:
  • Journal of Cerebral Blood Flow & Metabolism. - SAGE Publications. - 2004, vol. 24, no. 9, p. 1015-1024
Neurology
Clinical Neurology
Cardiology and Cardiovascular Medicine
English A new β-microprobe (βP) has been used to locally measure the time–concentration curve of a radiolabeled substance. The βP, analogous to positron emission tomography methodology, is useful for in vivo animal studies because it can acquire time–concentration curves with high temporal and spatial resolution. Using [18F]fluoro-2-deoxy-d-glucose and βP, we evaluated the reliability of the biologic parameters and we compared this method with the [14C]2-deoxy-d-glucose autoradiography. βP time–concentration curves in three regions of the brain were obtained from 24 rats. Four kinetic parameters (K1-k4) were estimated from 60-minute experimental periods using a three-compartment model. Best fits were obtained when the vascular fraction (Fv) was estimated simultaneously with the four kinetic parameters (K1-k4). The mean estimated Fv values were about 5.5% for the frontal cortex regions and 8.0% for the cerebellum. Correlation coefficients higher than 0.830 were observed between regional cerebral metabolic rates for glucose (rCMRglc) values obtained by βP and autoradiography. In addition, the βP-derived input function was similar to that obtained by manual sampling. Our findings show that reliable rCMRglc values can be obtained using βP.
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  • English
Open access status
bronze
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https://sonar.ch/global/documents/252348
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