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Evaluation of a new low sheath-flow interface for CE-MS.
Journal article

Evaluation of a new low sheath-flow interface for CE-MS.

  • González-Ruiz V School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva 4, Switzerland.
  • Codesido S Section of Mathematics, University of Geneva, Geneva 4, Switzerland.
  • Far J Laboratory of Mass Spectrometry, Chemistry Institute, University of Liège, Liège, Belgium.
  • Rudaz S School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva 4, Switzerland.
  • Schappler J School of Pharmaceutical Sciences, University of Geneva, University of Lausanne, Geneva 4, Switzerland.
  • 2016-02-06
Published in:
  • Electrophoresis. - 2016
CE-MS interface
Electrospray
Low sheath-flow
Electrophoresis, Capillary
Equipment Design
Limit of Detection
Reproducibility of Results
Spectrometry, Mass, Electrospray Ionization
English The current trend for increasing technical complexity in the field of CE-ESI-MS interfaces has incited for more accessible alternatives. In this work, a simple low sheath-flow ESI interface operating in the submicroliter nanospray regime without nebulizing gas assistance was evaluated. The use of sheath liquid enabled improving the ionization of the analytes, while the absence of nebulizing gas minimized sample dilution and loss of efficiency. After a rapid qualification, the effect of main operational parameters such as sheath liquid composition and flow rate, working distance and ESI potential was studied. Simulation of the mixing processes inside the Taylor cone proved its size to be of utmost importance in band broadening processes. As a proof of concept, the interface was eventually applied to a set of representative basic drugs analyzed by CE-TOF/MS. Limits of detection reached the 25-100 ppb range with suitable robustness and repeatability results. This design has demonstrated good performance while being simple and accessible to the user.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/252349
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