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Circulating human CD4 and CD8 T cells do not have large intracellular pools of CCR5
Journal article

Circulating human CD4 and CD8 T cells do not have large intracellular pools of CCR5

  • Pilch-Cooper, Heather A. Department of Medicine, Division of Infectious Diseases, Case Western Reserve University/University Hospitals/Case Medical Center, Cleveland, OH;
  • Sieg, Scott F. Department of Medicine, Division of Infectious Diseases, Case Western Reserve University/University Hospitals/Case Medical Center, Cleveland, OH;
  • Hope, Thomas J. Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL;
  • Koons, Ann Department of Cell and Molecular Biology, Feinberg School of Medicine, Northwestern University, Chicago, IL;
  • Escola, Jean-Michel Department of Structural Biology and Bioinformatics, Faculty of Medicine, University of Geneva, Geneva, Switzerland;
  • Offord, Robin Mintaka Foundation for Medical Research, Geneva, Switzerland;
  • Veazey, Ronald S. Tulane National Primate Research Center, Tulane University School of Medicine, Covington, LA;
  • Mosier, Donald E. Department of Immunology and Microbial Science, The Scripps Research Institute, La Jolla, CA;
  • Clagett, Brian Department of Medicine, Division of Infectious Diseases, Case Western Reserve University/University Hospitals/Case Medical Center, Cleveland, OH;
  • Medvik, Kathy Department of Medicine, Division of Infectious Diseases, Case Western Reserve University/University Hospitals/Case Medical Center, Cleveland, OH;
  • Jadlowsky, Julie K. Department of Molecular Biology and Microbiology, Case Western Reserve University School of Medicine, Cleveland, OH;
  • Chance, Mark R. Department of Physiology and Biophysics, Case Western Reserve University, Cleveland, OH; and
  • Kiselar, Janna G. Center for Proteomics and Bioinformatics, Case Western Reserve University, Cleveland, OH;
  • Hoxie, James A. Department of Medicine, University of Pennsylvania, Philadelphia, PA
  • Collman, Ronald G. Department of Medicine, University of Pennsylvania, Philadelphia, PA
  • Riddick, Nadeene E. Department of Medicine, University of Pennsylvania, Philadelphia, PA
  • Mercanti, Valentina Department of Structural Biology and Bioinformatics, Faculty of Medicine, University of Geneva, Geneva, Switzerland;
  • Hartley, Oliver Department of Structural Biology and Bioinformatics, Faculty of Medicine, University of Geneva, Geneva, Switzerland;
  • Lederman, Michael M. Department of Medicine, Division of Infectious Diseases, Case Western Reserve University/University Hospitals/Case Medical Center, Cleveland, OH;
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Published in:
  • Blood. - American Society of Hematology. - 2011, vol. 118, no. 4, p. 1015-1019
Immunology
Cell Biology
Biochemistry
Hematology
English AbstractCC Chemokine Receptor 5 (CCR5) is an important mediator of chemotaxis and the primary coreceptor for HIV-1. A recent report by other researchers suggested that primary T cells harbor pools of intracellular CCR5. With the use of a series of complementary techniques to measure CCR5 expression (antibody labeling, Western blot, quantitative reverse transcription polymerase chain reaction), we established that intracellular pools of CCR5 do not exist and that the results obtained by the other researchers were false-positives that arose because of the generation of irrelevant binding sites for anti-CCR5 antibodies during fixation and permeabilization of cells.
Language
  • English
Open access status
green
Identifiers
Persistent URL
https://sonar.ch/global/documents/252430
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