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Mille viae in eukaryotic mRNA decapping.
Journal article

Mille viae in eukaryotic mRNA decapping.

  • Valkov E Department of Biochemistry, Max Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tübingen, Germany.
  • Jonas S Institute of Biochemistry, ETH Zürich, Otto-Stern Weg 3, 8093 Zürich, Switzerland. Electronic address: stefanie.jonas@bc.biol.ethz.ch.
  • Weichenrieder O Department of Biochemistry, Max Planck Institute for Developmental Biology, Spemannstrasse 35, 72076 Tübingen, Germany. Electronic address: oliver.weichenrieder@tuebingen.mpg.de.
  • 2017-06-08
Published in:
  • Current opinion in structural biology. - 2017
Catalysis
Endoribonucleases
Eukaryota
Hydrolysis
Models, Molecular
Molecular Conformation
RNA Caps
RNA Stability
RNA, Messenger
Structure-Activity Relationship
English Cellular mRNA levels are regulated via rates of transcription and decay. Since the removal of the mRNA 5'-cap by the decapping enzyme DCP2 is generally an irreversible step towards decay, it requires regulation. Control of DCP2 activity is likely effected by two interdependent means: by conformational control of the DCP2-DCP1 complex, and by assembly control of the decapping network, an array of mutually interacting effector proteins. Here, we compare three recent and conformationally distinct crystal structures of the DCP2-DCP1 decapping complex in the presence of substrate analogs and decapping enhancers and we discuss alternative substrate recognition modes for the catalytic domain of DCP2. Together with structure-based insight into decapping network assembly, we propose that DCP2-mediated decapping follows more than one path.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/253694
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