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Study of peptide on-line complexation with transition-metal ions generated from sacrificial electrodes in thin-chip polymer microsprays.

  • Rohner TC Laboratoire d'Electrochimie Physique et Analytique, Ecole Polytechnique Fédérale de Lausanne, 1015 Lausanne, Switzerland.
  • Girault HH
  • 2005-04-09
Published in:
  • Rapid communications in mass spectrometry : RCM. - 2005
Angiotensin II
Copper
Electrodes
Humans
Iron
Mass Spectrometry
Models, Chemical
Nickel
Peptides
Polymers
Transition Elements
Zinc
English A miniaturized polymer electrospray-type interface is used to study metal-ion chelation with model peptides. Taking advantage of the intrinsic electrochemical behavior of electrospray, a sacrificial electrode is used to generate at the same time electrospray and transition-metal ions coming from the anodic dissolution of the electrode. The microspray interface provides enhanced mass transport due to its small dimensions, increasing the yield of possible reactions, in particular complex formation. Transition-metal electrodes, e.g. copper, zinc, nickel, iron and silver, are used to obtain on-line complexation with model peptides. It is demonstrated that the use of in-reservoir sacrificial electrodes is an efficient way to generate metal ions in order to form and study complexes with peptides, avoiding the addition of metallic salts.
Language
  • English
Open access status
green
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Persistent URL
https://sonar.ch/global/documents/25506
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