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The Hordeum bulbosum 25S-18S rDNA region: comparison with Hordeum vulgare and other Triticeae.
Journal article

The Hordeum bulbosum 25S-18S rDNA region: comparison with Hordeum vulgare and other Triticeae.

  • Georgiev O Institute of Molecular Life Sciences, University Zurich-Irchel, Winterthurer Str. 190, CH-8057 Zurich, Switzerland.
  • Mishev K Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, 1113 Sofia, Bulgaria.
  • Krasnikova M Department of Genetics, Faculty of Biology, St. Kl. Ohridsky University of Sofia, 8 Dragan Tsankov bld., 1164 Sofia, Bulgaria.
  • Kitanova M Department of Genetics, Faculty of Biology, St. Kl. Ohridsky University of Sofia, 8 Dragan Tsankov bld., 1164 Sofia, Bulgaria.
  • Dimitrova A Institute of Plant Physiology and Genetics, Bulgarian Academy of Sciences, Acad. G. Bonchev Str., Bl. 21, 1113 Sofia, Bulgaria, Phone: +359 2 9792677, Fax: +359 2 9785516, E-mail:annadimitrova0@gmail.com.
  • Karagyozov L Department of Genetics, Faculty of Biology, St. Kl. Ohridsky University of Sofia, 8 Dragan Tsankov bld., 1164 Sofia, Bulgaria.
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  • 2019-08-18
Published in:
  • Zeitschrift fur Naturforschung. C, Journal of biosciences. - 2019
Hordeum
external transcribed spacer
intergenic spacer
promoter
rDNA
Base Pairing
Base Sequence
Cell Nucleolus
DNA, Ribosomal
DNA, Ribosomal Spacer
Genes, Plant
Hordeum
Poaceae
Promoter Regions, Genetic
Repetitive Sequences, Nucleic Acid
Transcription Initiation Site
English Hordeum vulgare and Hordeum bulbosum are two closely related barley species, which share a common H genome. H. vulgare has two nucleolar organizer regions (NORs), while the NOR of H. bulbosum is only one. We sequenced the 2.5 kb 25S-18S region in the rDNA of H. bulbosum and compared it to the same region in H. vulgare as well as to the other Triticeae. The region includes an intergenic spacer (IGS) with a number of subrepeats, a promoter, and an external transcribed spacer (5'ETS). The IGS of H. bulbosum downstream of 25S rRNA contains two 143-bp repeats and six 128-bp repeats. In contrast, the IGS in H. vulgare contains an array of seven 79-bp repeats and a varying number of 135-bp repeats. The 135-bp repeats in H. vulgare and the 128-bp repeats in H. bulbosum show similarity. Compared to H. vulgare, the 5'ETS of H. bulbosum is shorter. Additionally, the 5'ETS regions in H. bulbosum and H. vulgare diverged faster than in other Triticeae genera. Alignment of the Triticeae promoter sequences suggests that in Hordeum, as in diploid Triticum, transcription starts with guanine and not with adenine as it is in many other plants.
Language
  • English
Open access status
closed
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Persistent URL
https://sonar.ch/global/documents/258097
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