The Methods of Choice for Extracellular Vesicles (EVs) Characterization.
- Szatanek R Department of Clinical Immunology, Institute of Paediatrics, Jagiellonian University Medical College, 30-663 Krakow, Poland. rafal.szatanek@uj.edu.pl.
- Baj-Krzyworzeka M Department of Clinical Immunology, Institute of Paediatrics, Jagiellonian University Medical College, 30-663 Krakow, Poland. mibaj@cyf-kr.edu.pl.
- Zimoch J Tissue Biology Research Unit, Department of Surgery, University Children's Hospital Zurich, 8032 Zurich, Switzerland. jakub.zimoch@kispi.uzh.ch.
- Lekka M Department of Research of Biophysical Microstructure, Institute of Nuclear Physics Polish Academy of Sciences, 31-342 Krakow, Poland. malgorzata.lekka@gmail.com.
- Siedlar M Department of Clinical Immunology, Institute of Paediatrics, Jagiellonian University Medical College, 30-663 Krakow, Poland. misiedla@cyf-kr.edu.pl.
- Baran J Department of Clinical Immunology, Institute of Paediatrics, Jagiellonian University Medical College, 30-663 Krakow, Poland. mibaran@cyf-kr.edu.pl.
- 2017-05-31
Published in:
- International journal of molecular sciences. - 2017
atomic force microscopy (AFM)
cryo-electron microscopy (Cryo-EM)
dynamic light scattering (DLS)
exosomes
extracellular vesicles (EVs)
flow cytometry
microvesicles (MVs)
nanoparticle tracking analysis (NTA)
stimulated emission depletion microscopy (STED)
transmission electron microscopy (TEM)
Animals
Cell-Derived Microparticles
Cryoelectron Microscopy
Exosomes
Extracellular Vesicles
Flow Cytometry
Humans
Microscopy, Atomic Force
Microscopy, Electron, Transmission
English
In recent years, extracellular vesicles (EVs) have become a subject of intense study. These membrane-enclosed spherical structures are secreted by almost every cell type and are engaged in the transport of cellular content (cargo) from parental to target cells. The impact of EVs transfer has been observed in many vital cellular processes including cell-to-cell communication and immune response modulation; thus, a fast and precise characterization of EVs may be relevant for both scientific and diagnostic purposes. In this review, the most popular analytical techniques used in EVs studies are presented with the emphasis on exosomes and microvesicles characterization.
- Language
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- English
- Open access status
- gold
- Identifiers
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- DOI 10.3390/ijms18061153
- PMID 28555055
- Persistent URL
- https://sonar.ch/global/documents/258262
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