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Mechanistic insights into mRNA 3'-end processing.
Journal article

Mechanistic insights into mRNA 3'-end processing.

  • Kumar A MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom.
  • Clerici M Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.
  • Muckenfuss LM Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland.
  • Passmore LA MRC Laboratory of Molecular Biology, Cambridge CB2 0QH, United Kingdom. Electronic address: passmore@mrc-lmb.cam.ac.uk.
  • Jinek M Department of Biochemistry, University of Zurich, Winterthurerstrasse 190, CH-8057 Zurich, Switzerland. Electronic address: jinek@bioc.uzh.ch.
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  • 2019-09-10
Published in:
  • Current opinion in structural biology. - 2019
3' Untranslated Regions
Binding Sites
Eukaryota
Models, Molecular
Polyadenylation
Protein Binding
RNA Processing, Post-Transcriptional
RNA, Messenger
RNA-Binding Proteins
Structure-Activity Relationship
English The polyadenosine (poly(A)) tail found on the 3'-end of almost all eukaryotic mRNAs is important for mRNA stability and regulation of translation. mRNA 3'-end processing occurs co-transcriptionally and involves more than 20 proteins to specifically recognize the polyadenylation site, cleave the pre-mRNA, add a poly(A) tail, and trigger transcription termination. The polyadenylation site (PAS) defines the end of the 3'-untranslated region (3'-UTR) and, therefore, selection of the cleavage site is a critical event in regulating gene expression. Integrated structural biology approaches including biochemical reconstitution of multi-subunit complexes, cross-linking mass spectrometry, and structural analyses by X- ray crystallography and single-particle electron cryo-microscopy (cryoEM) have enabled recent progress in understanding the molecular mechanisms of the mRNA 3'-end processing machinery. Here, we describe new molecular insights into pre-mRNA recognition, cleavage and polyadenylation.
Language
  • English
Open access status
hybrid
Identifiers
Persistent URL
https://sonar.ch/global/documents/258750
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