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Isolation and Culture of Human Dermal Fibroblasts.
Journal article

Isolation and Culture of Human Dermal Fibroblasts.

  • Kisiel MA Occupational and Environmental Medicine, Medical Sciences, Uppsala University Hospital, Uppsala, Sweden.
  • Klar AS Tissue Biology Research Unit, University Children's Hospital Zurich, University of Zurich, Zurich, Switzerland. agnes.klar@kispi.uzh.ch.
  • 2019-06-01
Published in:
  • Methods in molecular biology (Clifton, N.J.). - 2019
Collagen
Dermis
Fibroblasts
Organotypic culture
Regenerative medicine
Skin
Skin equivalent
Tissue engineering
Adult
Cell Culture Techniques
Cell Separation
Collagen
Dermis
Fibroblasts
Humans
Regenerative Medicine
Tissue Engineering
English Dermal fibroblasts are the main cell type present in skin connective tissue (dermis). Fibroblasts interact with epidermal cells during hair development and in interfollicular skin. Moreover, they play an essential role during cutaneous wound healing and in bioengineering of skin. Hence, culture of primary fibroblast is gaining in importance. In addition, fibroblasts established from skin biopsies provide a powerful tool for investigating normal skin physiology or specific disease states. In this chapter, detailed procedures for establishing and maintaining primary cultures of adult human dermal fibroblasts are described.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/260920
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