Electron microscopy.
- Luder HU Department of Oral Biology, Center of Dental Medicine, University of Zurich, Zurich, Switzerland. hansulrich.luder@zzm.uzh.ch
- Amstad-Jossi M
- 2012-05-09
Published in:
- Methods in molecular biology (Clifton, N.J.). - 2012
Animals
Incisor
Mice
Microscopy, Atomic Force
Microscopy, Electron, Transmission
Microtomy
Tissue Embedding
English
Correlative light (LM) and transmission electron microscopic (TEM) analysis is useful, if ultrastructural details of cells need to be related to functional aspects which can only be examined at the LM level. The first protocol presented here introduces a relatively simple way of obtaining TEM images which, on the one hand, reveal ultrastructural details of individual cells and, on the other hand, are large enough to allow a correlation with light micrographs. The second protocol describes a technique for estimating mineral densities of hard tissues using backscattered electron images obtained with a scanning electron microscope. This technique can be used to analyze the mineralization processes which occur throughout tooth formation.
- Language
-
- English
- Open access status
- green
- Identifiers
-
- DOI 10.1007/978-1-61779-860-3_9
- PMID 22566049
- Persistent URL
- https://sonar.ch/global/documents/262955
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