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Killing Bacteria with Cytotoxic Effector Proteins of Human Killer Immune Cells: Granzymes, Granulysin, and Perforin.
Journal article

Killing Bacteria with Cytotoxic Effector Proteins of Human Killer Immune Cells: Granzymes, Granulysin, and Perforin.

  • León DL Unit of Anatomy, Department of Medicine, University of Fribourg, Rue A.-Gockel 1, 1700, Fribourg, Switzerland.
  • Fellay I Unit of Anatomy, Department of Medicine, University of Fribourg, Rue A.-Gockel 1, 1700, Fribourg, Switzerland.
  • Mantel PY Unit of Anatomy, Department of Medicine, University of Fribourg, Rue A.-Gockel 1, 1700, Fribourg, Switzerland.
  • Walch M Unit of Anatomy, Department of Medicine, University of Fribourg, Rue A.-Gockel 1, 1700, Fribourg, Switzerland. michael.walch@unifr.ch.
  • 2016-12-04
Published in:
  • Methods in molecular biology (Clifton, N.J.). - 2017
Antibacterial activity
Cell-mediated cytotoxicity
Granulysin
Granzymes
Immune serine proteases
Anti-Bacterial Agents
Antigens, Differentiation, T-Lymphocyte
Bacteria
Cell Line
Cytotoxicity, Immunologic
Dose-Response Relationship, Drug
Extracellular Space
Granzymes
Humans
Intracellular Space
Killer Cells, Natural
Perforin
T-Lymphocytes, Cytotoxic
English Bacterial pathogens represent a constant threat to human health that was exacerbated in recent years by a dramatic increase of strains resistant to last resort antibiotics. The immune system of higher vertebrates generally evolved several efficient innate and adaptive mechanisms to fight ubiquitous bacterial pathogens. Among those mechanisms, immune proteases were recognized to contribute essentially to antibacterial immune defense. The effector serine proteases of the adaptive immune system, the granzymes, exert potent antimicrobial activity when they are delivered into the bacterial cytosol by prokaryotic membrane disrupting proteins, such as granulysin.In this chapter, we are detailing experimental protocols to study the synergistic cytotoxic effects of human granzymes and granulysin on extracellular as well as on intracellular bacterial pathogens in vitro. In addition, we provide a simple and fast-forward method to biochemically purify native cytotoxic effector molecules necessary to perform this kind of investigations.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/264425
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