Journal article
Ca2(+)-binding site of carp parvalbumin recognized by monoclonal antibody.
- Tinner R Biochemisches Institut, Universität Zürich, Switzerland.
- Oertle M
- Heizmann CW
- Bosshard HR
- 1990-01-01
Published in:
- Cell calcium. - 1990
Amino Acid Sequence
Animals
Antibodies, Monoclonal
Binding Sites
Calcium
Carps
Cyprinidae
Epitopes
Molecular Sequence Data
Muscle Proteins
Parvalbumins
Protein Conformation
English
Monoclonal antibody 235 which was used for immunohistochemical staining of parvalbumin in tissue sections partially protects Lys-54 of carp muscle parvalbumin from reaction with acetic anhydride in the parvalbumin-antibody complex. Lys-54 is located in the CD-loop of parvalbumin and is flanked by the Ca2(+)-ligands Asp-53 and Ser-55 of the Ca2(+)-site I. Another monoclonal antibody against carp parvalbumin, mca 239, partially protects lysine residues 27, 32, 87 and 107, indicating that this antibody is directed against a discontinuous epitope distant from the two Ca2(+)-binding sites of parvalbumin.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1016/0143-4160(90)90045-v
- PMID 1690078
- Persistent URL
- https://sonar.ch/global/documents/264884
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