Journal article
Fluorescence correlation spectroscopy.
- Ries J Institute of Biochemistry, ETH Zurich, Zurich, Switzerland.
- Schwille P
- 2012-03-15
Published in:
- BioEssays : news and reviews in molecular, cellular and developmental biology. - 2012
Fluorescent Dyes
Models, Theoretical
Molecular Imaging
Proteins
Solutions
Spectrometry, Fluorescence
Whole Body Imaging
English
Fluorescence correlation spectroscopy (FCS) is a powerful technique to measure concentrations, mobilities, and interactions of fluorescent biomolecules. It can be applied to various biological systems such as simple homogeneous solutions, cells, artificial, or cellular membranes and whole organisms. Here, we introduce the basic principle of FCS, discuss its application to biological questions as well as its limitations and challenges, present an overview of novel technical developments to overcome those challenges, and conclude with speculations about the future applications of fluorescence fluctuation spectroscopy.
- Language
-
- English
- Open access status
- closed
- Identifiers
-
- DOI 10.1002/bies.201100111
- PMID 22415816
- Persistent URL
- https://sonar.ch/global/documents/265563
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