Triplex real-time PCR method for the qualitative detection of European and American foulbrood in honeybee.

Dainat B; Grossar D; Ecoffey B; Haldemann C

doi:10.1016/j.mimet.2018.01.018

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Journal article

Triplex real-time PCR method for the qualitative detection of European and American foulbrood in honeybee.

Dainat B Agroscope, Schwarzenburgstrasse 161, CH-3003 Bern, Switzerland. Electronic address: benjamin.dainat@agroscope.admin.ch.
Grossar D Agroscope, Schwarzenburgstrasse 161, CH-3003 Bern, Switzerland; Department of Ecology and Evolution, University of Lausanne, Biophore, UNIL-Sorge, CH-1015 Lausanne, Switzerland.
Ecoffey B Agroscope, Schwarzenburgstrasse 161, CH-3003 Bern, Switzerland.
Haldemann C Agroscope, Schwarzenburgstrasse 161, CH-3003 Bern, Switzerland.

2018-02-03

Published in:

Journal of microbiological methods. - 2018

Multiplex Polymerase Chain Reaction

English The bacteria Melissococcus plutonius and Paenibacillus larvae, causative agents of respectively European and American foulbrood, damage honeybee health worldwide. Here, we present a specific and sensitive qualitative triplex real-time PCR method to detect simultaneously those microbial agents and a honeybee gene, validated through a study involving 7 laboratories through Europe.

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