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Triplex real-time PCR method for the qualitative detection of European and American foulbrood in honeybee.
Journal article

Triplex real-time PCR method for the qualitative detection of European and American foulbrood in honeybee.

  • Dainat B Agroscope, Schwarzenburgstrasse 161, CH-3003 Bern, Switzerland. Electronic address: benjamin.dainat@agroscope.admin.ch.
  • Grossar D Agroscope, Schwarzenburgstrasse 161, CH-3003 Bern, Switzerland; Department of Ecology and Evolution, University of Lausanne, Biophore, UNIL-Sorge, CH-1015 Lausanne, Switzerland.
  • Ecoffey B Agroscope, Schwarzenburgstrasse 161, CH-3003 Bern, Switzerland.
  • Haldemann C Agroscope, Schwarzenburgstrasse 161, CH-3003 Bern, Switzerland.
  • 2018-02-03
Published in:
  • Journal of microbiological methods. - 2018
Apis mellifera
Diagnosis
Melissococcus plutonius
Multiplex real-time PCR
Paenibacillus larvae
Americas
Animal Diseases
Animals
Bees
DNA, Bacterial
Enterococcaceae
Europe
Multiplex Polymerase Chain Reaction
Paenibacillus larvae
English The bacteria Melissococcus plutonius and Paenibacillus larvae, causative agents of respectively European and American foulbrood, damage honeybee health worldwide. Here, we present a specific and sensitive qualitative triplex real-time PCR method to detect simultaneously those microbial agents and a honeybee gene, validated through a study involving 7 laboratories through Europe.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/266461
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