Biomimetic Conditioning of Human Dentin Using Citric Acid.
- Ivica A Department of Cranio-Maxillofacial and Oral Surgery, Oral Biotechnology and Bioengineering, University of Zurich, Zurich, Switzerland.
- Zehnder M Department of Preventive Dentistry, Periodontology and Cariology, University of Zurich, Zurich, Switzerland.
- Mateos JM Department of Center for Microscopy and Image Analysis, University of Zurich, Zurich, Switzerland.
- Ghayor C Department of Cranio-Maxillofacial and Oral Surgery, Oral Biotechnology and Bioengineering, University of Zurich, Zurich, Switzerland.
- Weber FE Department of Cranio-Maxillofacial and Oral Surgery, Oral Biotechnology and Bioengineering, University of Zurich, Zurich, Switzerland. Electronic address: franz.weber@zzm.uzh.ch.
- 2018-11-19
Published in:
- Journal of endodontics. - 2019
Bioengineering
cell-matrix interactions
dentin
growth factor(s)
regeneration
Biomimetics
Cell Adhesion
Cell Movement
Cell Survival
Cells, Cultured
Citric Acid
Dentin
Edetic Acid
Humans
Mesenchymal Stem Cells
Microscopy, Confocal
Molecular Imaging
Regenerative Endodontics
Tissue Conditioning, Dental
Transforming Growth Factor beta1
English
INTRODUCTION
In carious teeth, transforming growth factor beta 1 (TGF-β1) is released from the dentin matrix and possibly activated in an acidic environment. Conversely, EDTA solutions with a neutral to slightly alkaline pH are used in clinics to promote cell homing in regenerative endodontic procedures. We hypothesized that citric acid (CA) might be more beneficial.
METHODS
TGF-β1 release from human dentin disks conditioned with either 10% CA (pH = 2) or 17% EDTA (pH = 8) and the behavior of human stem cells toward such pretreated dentin were studied. The protein concentration in conditioning solutions after 10 minutes of dentin exposure was determined using a pH-independent slot blot technique.
RESULTS
There was a 5-fold higher concentration of the target protein in CA (382 ± 30 ng/disk) compared with EDTA (66 ± 3 ng/disk, P < .005). Using confocal laser scanning microscopy on immunofluorescent-labeled disks, we identified a high density of TGF-β1 in peritubular dentin after CA treatment. A migration assay showed that CA conditioning attracted significantly more stem cells toward the dentin after 24 hours compared with EDTA (P < .05) or phosphate-buffered saline (P < .005). To investigate whether the cell response to these dentin surfaces could be affected by different pretreatments, we cultured stem cells on conditioned dentin disks and found that CA had a significantly (P < .05) better effect than EDTA on cell attachment and cell survival.
CONCLUSIONS
CA conditioning could be useful and may have significant benefits over current treatments.
In carious teeth, transforming growth factor beta 1 (TGF-β1) is released from the dentin matrix and possibly activated in an acidic environment. Conversely, EDTA solutions with a neutral to slightly alkaline pH are used in clinics to promote cell homing in regenerative endodontic procedures. We hypothesized that citric acid (CA) might be more beneficial.
METHODS
TGF-β1 release from human dentin disks conditioned with either 10% CA (pH = 2) or 17% EDTA (pH = 8) and the behavior of human stem cells toward such pretreated dentin were studied. The protein concentration in conditioning solutions after 10 minutes of dentin exposure was determined using a pH-independent slot blot technique.
RESULTS
There was a 5-fold higher concentration of the target protein in CA (382 ± 30 ng/disk) compared with EDTA (66 ± 3 ng/disk, P < .005). Using confocal laser scanning microscopy on immunofluorescent-labeled disks, we identified a high density of TGF-β1 in peritubular dentin after CA treatment. A migration assay showed that CA conditioning attracted significantly more stem cells toward the dentin after 24 hours compared with EDTA (P < .05) or phosphate-buffered saline (P < .005). To investigate whether the cell response to these dentin surfaces could be affected by different pretreatments, we cultured stem cells on conditioned dentin disks and found that CA had a significantly (P < .05) better effect than EDTA on cell attachment and cell survival.
CONCLUSIONS
CA conditioning could be useful and may have significant benefits over current treatments.
- Language
-
- English
- Open access status
- hybrid
- Identifiers
-
- DOI 10.1016/j.joen.2018.09.015
- PMID 30448020
- Persistent URL
- https://sonar.ch/global/documents/266682
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