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NtrC-dependent control of exopolysaccharide synthesis and motility in Burkholderia cenocepacia H111.

  • Liu Y Department of Plant and Microbial Biology, University of Zurich, Zurich, Switzerland.
  • Lardi M Department of Plant and Microbial Biology, University of Zurich, Zurich, Switzerland.
  • Pedrioli A Department of Plant and Microbial Biology, University of Zurich, Zurich, Switzerland.
  • Eberl L Department of Plant and Microbial Biology, University of Zurich, Zurich, Switzerland.
  • Pessi G Department of Plant and Microbial Biology, University of Zurich, Zurich, Switzerland.
  • 2017-06-30
Published in:
  • PloS one. - 2017
Animals
Bacterial Proteins
Biofilms
Burkholderia cenocepacia
Caenorhabditis elegans
Mutation
Nitrogen
Polysaccharides
Real-Time Polymerase Chain Reaction
Sequence Analysis, RNA
Virulence
English Burkholderia cenocepacia is a versatile opportunistic pathogen that survives in a wide variety of environments, which can be limited in nutrients such as nitrogen. We have previously shown that the sigma factor σ54 is involved in the control of nitrogen assimilation and virulence in B. cenocepacia H111. In this work, we investigated the role of the σ54 enhancer binding protein NtrC in response to nitrogen limitation and in the pathogenicity of H111. Of 95 alternative nitrogen sources tested the ntrC showed defects in the utilisation of nitrate, urea, L-citrulline, acetamide, DL-lactamide, allantoin and parabanic acid. RNA-Seq and phenotypic analyses of an ntrC mutant strain showed that NtrC positively regulates two important phenotypic traits: exopolysaccharide (EPS) production and motility. However, the ntrC mutant was not attenuated in C. elegans virulence.
Language
  • English
Open access status
gold
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Persistent URL
https://sonar.ch/global/documents/266716
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