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Staurosporine but not chelerythrine inhibits regeneration in hippocampal organotypic cultures.
Journal article

Staurosporine but not chelerythrine inhibits regeneration in hippocampal organotypic cultures.

  • 1997-11-05
Published in:
  • Synapse (New York, N.Y.). - 1997
Alkaloids
Animals
Animals, Newborn
Benzophenanthridines
Electrophysiology
Enzyme Inhibitors
Hippocampus
Immunohistochemistry
Long-Term Potentiation
Nerve Fibers
Nerve Regeneration
Neurites
Neuronal Plasticity
Organ Culture Techniques
Phenanthridines
Protein Kinase C
Rats
Rats, Wistar
Staurosporine
English A mechanical lesion in hippocampal organotypic cultures is followed by a recovery process involving scar formation, sprouting of fibres and formation of new functional synapses. Here we tested the effect of staurosporine and chelerythrine, two protein kinase C (PKC) inhibitors, on this lesion-induced neurite outgrowth of Shaffer collaterals. At a concentration of 1 microM, staurosporine delayed functional recovery assessed by measuring synaptic field potentials across the lesion, without altering synaptic transmission on nonlesioned cultures. Immunostaining carried out by using antibodies directed against neurofilament proteins showed that there was a marked reduction in the number of regenerating fibres crossing the lesion. In contrast to this, chelerythrine (50 microM) did not prevent functional recovery, although it affected synaptic transmission and plasticity at this concentration. We conclude that the inhibition of sprouting produced by staurosporine is independent of its blockade of PKC-mediated phosphorylation mechanisms.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/26674
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