Effects of small pulsed nanocurrents on cell viability in vitro and in vivo: implications for biomedical electrodes.
- Gabi M Laboratory of Biosensors and Bioelectronics, ETH Zurich, Zurich, Switzerland.
- Bullen ME
- Agarkova I
- Schmidt D
- Schoenauer R
- Brokopp C
- Emmert MY
- Larmagnac A
- Sannomiya T
- Weber B
- Wilhelm MJ
- Vörös J
- Hoerstrup SP
- 2010-08-31
Published in:
- Biomaterials. - 2010
Animals
Biomedical Technology
Cell Death
Cell Survival
Electric Impedance
Electricity
Electrodes
Nanostructures
Prosthesis Implantation
Rats
Rats, Sprague-Dawley
English
Using a custom-built, implantable pulse generator, we studied the effects of small pulsed currents on the viability on rat aortic-derived cells (RAOC) in vitro. The pulsed currents (0.37A/m(2)) underwent apoptosis within 24h as shown by the positive staining for cleaved caspase-3 and classically apoptotic morphology. Based on these findings, we examined the effects of nanocurrents in vivo. The pulse generator was implanted subcutaneously in the rat model. The electrode|tissue interface histology revealed no difference between the active platinum surface and the neighboring control surface, however we found a large difference between electrodes that were functional during the entire experiment and non-active electrodes. These non-active electrodes showed an increase in impedance at higher frequencies 21 days post-implantation, whereas working electrodes retained their impedance value for the entire experiment. These results indicate that applied currents can reduce the impedance of implanted electrodes.
- Language
-
- English
- Open access status
- green
- Identifiers
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- DOI 10.1016/j.biomaterials.2010.07.092
- PMID 20800892
- Persistent URL
- https://sonar.ch/global/documents/266840
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