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A Rapid TALEN Assembly Protocol.
Journal article

A Rapid TALEN Assembly Protocol.

  • Akmammedov A Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule Zürich, Mattenstrasse 26, 4058, Basel, Switzerland. arslan.akmammedov@bsse.ethz.ch.
  • Katsuyama T Department of Genetics, Graduate School of Pharmaceutical Sciences, The University of Tokyo, Hongo 7-3-1 Bunkyo-ku, 113-8654, Tokyo, Japan.
  • Paro R Department of Biosystems Science and Engineering, Eidgenössische Technische Hochschule Zürich, Mattenstrasse 26, 4058, Basel, Switzerland.
  • 2016-09-24
Published in:
  • Methods in molecular biology (Clifton, N.J.). - 2016
Genome engineering
Site-specific gene integration
TALEN
DNA Breaks, Double-Stranded
DNA End-Joining Repair
Genetic Engineering
Homologous Recombination
Mutagenesis, Site-Directed
Transcription Activator-Like Effector Nucleases
English Owing to their modular and highly specific DNA recognition mode, transcription activator-like effector nucleases (TALENs) have been rapidly adopted by the scientific community for the purpose of generating site-specific double-strand breaks (DSBs) on a DNA molecule. A pair of TALENs can be used to produce random insertions or deletions of various lengths via nonhomologous end-joining or together with a homologous donor DNA to induce precise sequence alterations by homologous recombination (HR). Here, we describe a method for TALEN assembly (easyT) and a strategy for genome engineering via HR.
Language
  • English
Open access status
closed
Identifiers
Persistent URL
https://sonar.ch/global/documents/267461
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