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A Versatile Biosynthetic Hydrogel Platform for Engineering of Tissue Analogues.

  • Klotz BJ Department of Oral and Maxillofacial Surgery and Special Dental Care, University Medical Center Utrecht, Utrecht University, 3508 GA, Utrecht, the Netherlands.
  • Oosterhoff LA Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 3508 TC, Utrecht, the Netherlands.
  • Utomo L Department of Oral and Maxillofacial Surgery and Special Dental Care, University Medical Center Utrecht, Utrecht University, 3508 GA, Utrecht, the Netherlands.
  • Lim KS Department of Orthopaedic Surgery and Musculoskeletal Medicine, Centre for Bioengineering and Nanomedicine, University of Otago, Christchurch, 8011, New Zealand.
  • Vallmajo-Martin Q Department of Obstetrics, University Hospital Zurich, University of Zurich, 8091, Zurich, Switzerland.
  • Clevers H Hubrecht Institute, Royal Netherlands Academy of Arts and Sciences, University Medical Center Utrecht, 3584 CT, Utrecht, the Netherlands.
  • Woodfield TBF Department of Orthopaedic Surgery and Musculoskeletal Medicine, Centre for Bioengineering and Nanomedicine, University of Otago, Christchurch, 8011, New Zealand.
  • Rosenberg AJWP Department of Oral and Maxillofacial Surgery and Special Dental Care, University Medical Center Utrecht, Utrecht University, 3508 GA, Utrecht, the Netherlands.
  • Malda J Regenerative Medicine Utrecht, 3584 CT, Utrecht, the Netherlands.
  • Ehrbar M Department of Obstetrics, University Hospital Zurich, University of Zurich, 8091, Zurich, Switzerland.
  • Spee B Department of Clinical Sciences of Companion Animals, Faculty of Veterinary Medicine, Utrecht University, 3508 TC, Utrecht, the Netherlands.
  • Gawlitta D Department of Oral and Maxillofacial Surgery and Special Dental Care, University Medical Center Utrecht, Utrecht University, 3508 GA, Utrecht, the Netherlands.
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  • 2019-08-13
Published in:
  • Advanced healthcare materials. - 2019
FXIII
Matrigel
gelatin
liver organoids
osteogenesis
polyethylene glycol
vasculogenesis
Animals
Biocompatible Materials
Bone and Bones
Catalysis
Cell Differentiation
Cell Survival
Collagen
Culture Media
Drug Combinations
Humans
Hydrogels
Laminin
Liver
Mesenchymal Stem Cells
Organoids
Peptides
Polyethylene Glycols
Proteoglycans
Swine
Tissue Engineering
Tissue Scaffolds
English For creating functional tissue analogues in tissue engineering, stem cells require very specific 3D microenvironments to thrive and mature. Demanding (stem) cell types that are used nowadays can find such an environment in a heterogeneous protein mixture with the trade name Matrigel. Several variations of synthetic hydrogel platforms composed of poly(ethylene glycol) (PEG), which are spiked with peptides, have been recently developed and shown equivalence to Matrigel for stem cell differentiation. Here a clinically relevant hydrogel platform, based on PEG and gelatin, which even outperforms Matrigel when targeting 3D prevascularized bone and liver organoid tissue engineering models is presented. The hybrid hydrogel with natural and synthetic components stimulates efficient cell differentiation, superior to Matrigel models. Furthermore, the strength of this hydrogel lies in the option to covalently incorporate unmodified proteins. These results demonstrate how a hybrid hydrogel platform with intermediate biological complexity, when compared to existing biological materials and synthetic PEG-peptide approaches, can efficiently support tissue development from human primary cells.
Language
  • English
Open access status
hybrid
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Persistent URL
https://sonar.ch/global/documents/26925
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